Isolation of Plasma-membrane-bound Calcium/calmodulin-regulated Protein Kinase from Pea Using Western Blotting

Overview

Journal Planta

Specialty Biology

Date 2013 Nov 19

PMID 24241434

Citations 1

Authors

D P Blowers

A Hetherington

A Trewavas

Affiliations

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Abstract

Membranes isolated from pea buds contain protein-kinase activity which is greatly activated by low concentrations of calcium ions. This paper describes a simple purification of this enzyme with a novel means of detecting enzyme activity by Western blotting. The purified enzyme appears to autophosphorylate primarily on serine residues, is activated by bovine calmodulin and additional evidence from phase partitioning indicate most of this enzyme to be located in the plasma membrane.

Citing Articles

Calcium-dependent protein kinase from apple fruit membranes is calmodulin-independent but has calmodulin-like properties.

Battey N, Venis M Planta. 2013; 176(1):91-7.

PMID: 24220739 DOI: 10.1007/BF00392484.

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