Effect of Salicin on Gut Inflammation and on Selected Groups of Gut Microbiota in Dextran Sodium Sulfate Induced Mouse Model of Colitis

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2013 Nov 19

PMID 24240229

Citations 4

Authors

Nirmal Verma

Ravi Verma

Reena Kumari

Raju Ranjha

Jaishree Paul

Affiliations

Soon will be listed here.

Abstract

Objective And Design: This study was undertaken to evaluate the anti-inflammatory effect of the pure compound salicin on dextran sulfate sodium (DSS)-induced colitis in a mouse model and to quantify the major gut bacteria during the treatment.

Material Or Subjects: Experimental colitis was induced in Swiss albino mice by dissolving 2 % DSS in their drinking water for 7 days. Five mice were used in each group.

Treatment: Salicin (100 and 200 mg per body weight) was administered daily through oral gavage for 7 days.

Methods: Disease activity index (DAI), colon length, myeloperoxidase (MPO) assay, pro-inflammatory cytokine expression, histological changes and absolute number of gut microbiota were measured after treatment. Student's t test was applied for statistical analysis.

Results: Salicin significantly attenuated DSS-induced DAI scores, shortening of colon length and tissue MPO activity. Salicin administration also effectively and dose-dependently prevented pro-inflammatory cytokine expression in DSS-induced colitis mice. Histological examination indicated that salicin suppressed edema, mucosal damage and the loss of crypts induced by DSS. Oral administration of salicin in DSS-treated mice prevented loss of gut microbiota during the short period of treatment.

Conclusions: Salicin has an anti-inflammatory effect, and it may have therapeutic value in ameliorating inflammation during colitis.

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References

Malle E, Furtmuller P, Sattler W, Obinger C . Myeloperoxidase: a target for new drug development?. Br J Pharmacol. 2007; 152(6):838-54. PMC: 2078229. DOI: 10.1038/sj.bjp.0707358. View

Maxton D, Morris J, Whorwell P . More accurate diagnosis of irritable bowel syndrome by the use of 'non-colonic' symptomatology. Gut. 1991; 32(7):784-6. PMC: 1378996. DOI: 10.1136/gut.32.7.784. View

Talley N, Stanghellini V, Heading R, Koch K, Malagelada J, Tytgat G . Functional gastroduodenal disorders. Gut. 1999; 45 Suppl 2:II37-42. PMC: 1766695. DOI: 10.1136/gut.45.2008.ii37. View

Hedner T, Everts B . The early clinical history of salicylates in rheumatology and pain. Clin Rheumatol. 1998; 17(1):17-25. DOI: 10.1007/BF01450953. View

Breese E, Michie C, Nicholls S, Murch S, Williams C, Domizio P . Tumor necrosis factor alpha-producing cells in the intestinal mucosa of children with inflammatory bowel disease. Gastroenterology. 1994; 106(6):1455-66. DOI: 10.1016/0016-5085(94)90398-0. View

Sydora B, Albert E, Foshaug R, Doyle J, Churchill T, Fedorak R . Intravenous injection of endogenous microbial components abrogates DSS-induced colitis. Dig Dis Sci. 2011; 57(2):345-54. DOI: 10.1007/s10620-011-1878-5. View

Rojas-Cartagena C, Flores I, Appleyard C . Role of tumor necrosis factor receptors in an animal model of acute colitis. Cytokine. 2005; 32(2):85-93. DOI: 10.1016/j.cyto.2005.08.001. View

Kinoshita K, Hori M, Fujisawa M, Sato K, Ohama T, Momotani E . Role of TNF-alpha in muscularis inflammation and motility disorder in a TNBS-induced colitis model: clues from TNF-alpha-deficient mice. Neurogastroenterol Motil. 2006; 18(7):578-88. DOI: 10.1111/j.1365-2982.2006.00784.x. View

Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R . A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology. 1990; 98(3):694-702. DOI: 10.1016/0016-5085(90)90290-h. View

10.

Thompson-Chagoyan O, Maldonado J, Gil A . Aetiology of inflammatory bowel disease (IBD): role of intestinal microbiota and gut-associated lymphoid tissue immune response. Clin Nutr. 2005; 24(3):339-52. DOI: 10.1016/j.clnu.2005.02.009. View

11.

Seksik P, Sokol H, Lepage P, Vasquez N, Manichanh C, Mangin I . Review article: the role of bacteria in onset and perpetuation of inflammatory bowel disease. Aliment Pharmacol Ther. 2006; 24 Suppl 3:11-8. DOI: 10.1111/j.1365-2036.2006.03053.x. View

12.

Talero E, Sanchez-Fidalgo S, Calvo J, Motilva V . Galanin in the trinitrobenzene sulfonic acid rat model of experimental colitis. Int Immunopharmacol. 2006; 6(9):1404-12. DOI: 10.1016/j.intimp.2006.04.016. View

13.

Fiocchi C . Inflammatory bowel disease: etiology and pathogenesis. Gastroenterology. 1998; 115(1):182-205. DOI: 10.1016/s0016-5085(98)70381-6. View

14.

Linskens R, Huijsdens X, Savelkoul P, Vandenbroucke-Grauls C, Meuwissen S . The bacterial flora in inflammatory bowel disease: current insights in pathogenesis and the influence of antibiotics and probiotics. Scand J Gastroenterol Suppl. 2002; (234):29-40. DOI: 10.1080/003655201753265082. View

15.

Cooper H, Murthy S, Shah R, Sedergran D . Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 1993; 69(2):238-49. View

16.

Gonzalez-Rey E, Chorny A, Delgado M . Therapeutic action of ghrelin in a mouse model of colitis. Gastroenterology. 2006; 130(6):1707-20. DOI: 10.1053/j.gastro.2006.01.041. View

17.

Ghosh S, van Heel D, Playford R . Probiotics in inflammatory bowel disease: is it all gut flora modulation?. Gut. 2004; 53(5):620-2. PMC: 1774052. DOI: 10.1136/gut.2003.034249. View

18.

Dong L, Li J, Liu Y, Yue W, Luo X . Toll-like receptor 2 monoclonal antibody or/and Toll-like receptor 4 monoclonal antibody increase counts of Lactobacilli and Bifidobacteria in dextran sulfate sodium-induced colitis in mice. J Gastroenterol Hepatol. 2011; 27(1):110-9. DOI: 10.1111/j.1440-1746.2011.06839.x. View

19.

Guarner F, Malagelada J . Gut flora in health and disease. Lancet. 2003; 361(9356):512-9. DOI: 10.1016/S0140-6736(03)12489-0. View

20.

Verma R, Verma A, Ahuja V, Paul J . Real-time analysis of mucosal flora in patients with inflammatory bowel disease in India. J Clin Microbiol. 2010; 48(11):4279-82. PMC: 3020854. DOI: 10.1128/JCM.01360-10. View