Comparative Restriction Endonuclease Analysis and Molecular Cloning of Plastid DNAs from Wild Species and Cultivated Varieties of the Genus Beta (L.)

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 19

PMID 24240214

Citations 14

Authors

K Fritzsche

M Metzlaff

R Melzer

R Hagemann

Affiliations

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Abstract

A phyletic tree of the genus Beta has been constructed based on EcoRI and PstI plastid DNA restriction patterns of eight species from three sections of the genus. In contrast to the remarkable morphological variability of the varieties of B. vulgaris the restriction patterns of the plastid DNA of this species were found to be almost identical. The comparison of plastic DNAs of B. vulgaris crassa fertile and sterile lines with 13 different restriction enzymes revealed only a single fragment polymorphism in the HindIII patterns. Hybridization analyses in the plastidal rDNA region revealed an interesting loss of an EcoRI restriction site in all cultivated B. vulgaris varieties in contrast to wild species. The results of the construction of clone banks for SalI and BamHI fragments of plastid DNA from fertile B. vulgaris crassa are reported and difficulties in the cloning of specific fragments are discussed.

Citing Articles

Insights into the genetic relationships among plants of Beta section Beta using SNP markers.

Andrello M, Henry K, Devaux P, Verdelet D, Desprez B, Manel S Theor Appl Genet. 2017; 130(9):1857-1866.

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Comparison of chloroplast and mitochondrial DNA from five morphologically distinct Beta vulgaris cultivars: sugar beet, fodder beet, beet root, foliage beet, and Swiss chard.

Ecke W, Michaelis G Theor Appl Genet. 2013; 79(4):440-2.

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Plastid DNA diversity in natural populations of Beta maritima showing additional variation in sexual phenotype and mitochondrial DNA.

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Distribution and evolution of two satellite DNAs in the genus Beta.

Schmidt T, Jung C, Metzlaff M Theor Appl Genet. 2013; 82(6):793-9.

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A full set of monosomic addition lines in Beta vulgaris from Beta webbiana: morphology and isozyme markers.

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