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Persistent Circadian Rhythms in the Phosphorylation State of Phosphoenolpyruvate Carboxylase from Bryophyllum Fedtschenkoi Leaves and in Its Sensitivity to Inhibition by Malate

Overview
Journal Planta
Specialty Biology
Date 2013 Nov 16
PMID 24232972
Citations 20
Authors
G A Nimmo
M B Wilkins
C A Fewson
H G Nimmo
Affiliations
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Abstract

Phosphoenolpyruvate carboxylase (EC 4.1.1.31; PEPCase) from Bryophyllum fedtschenkoi leaves has previously been shown to exist in two forms in vivo. During the night the enzyme is phosphorylated and relatively insensitive to feedback inhibition by malate whereas during the day the enzyme is dephosphorylated and more sensitive to inhibition by malate. These properties of PEPCase have now been investigated in leaves maintained under constant conditions of temperature and lighting. When leaves were maintained in continuous darkness and CO2-free air at 15°C, PEPCase exhibited a persistent circadian rhythm of interconversion between the two forms. There was a good correlation between periods during which the leaves were fixing respiratory CO2 and periods during which PEPCase was in the form normally observed at night. When leaves were maintained in continuous light and normal air at 15°C, starting at the end of a night or the end of a day, a circadian rhythm of net uptake of CO2 was observed. Only when these constant conditions were applied at the end of a day was a circadian rhythm of interconversions between the two forms of PEPCase observed and the rhythms of enzyme interconversion and CO2 uptake did not correlate in phase or period.

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References
1.
Wilkins M . An endogenous rhythm in the rate of carbon dioxide output of Bryophyllum V. The dependence of rhythmicity upon aerobic metabolism. Planta. 2014; 72(1):66-77. DOI: 10.1007/BF00388146. View
2.
Brulfert J, Queiroz O . Photoperiodism and Crassulacean acid metabolism : III. Different characteristics of the photoperiod-sensitive and non-sensitive isoforms of phosphoenolpyruvate carboxylase and Crassulacean acid metabolism operation. Planta. 2013; 154(4):339-43. DOI: 10.1007/BF00393912. View
3.
Wilkns M . The circadian rhythm of carbon-dioxide metabolism in Bryophyllum: the mechanism of phase-shift induction by thermal stimuli. Planta. 2013; 157(5):471-780. DOI: 10.1007/BF00397205. View
4.
Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View
5.
Fischer A, Kluge M . Studies on carbon flow in Crassulacean acid metabolism during the initial light period. Planta. 2013; 160(2):121-8. DOI: 10.1007/BF00392860. View