Breakdown of Phosphatidylinositol in Soybean Callus

Overview

Journal Planta

Specialty Biology

Date 2013 Nov 16

PMID 24232553

Citations 3

Authors

R J Connett

D E Hanke

Affiliations

Soon will be listed here.

Abstract

We have investigated the breakdown of membrane-bound phosphatidylinositol (PI) in homogenates of soybean (Glycine max) callus. The breakdown of PI was stimulated by the detergent deoxycholate. At pH 7.0 and 1·gl(-1) of deoxycholate the loss of PI was rapid and extensive: more than 80% was broken down within 10 min. The breakdown of PI was also stimulated by millimolar concentrations of Ca(2+). The products of breakdown of added PI (purified from soybean callus) in this system were identified from their chromatographic mobilities as 1,2-diacylglycerol, myo-inositol 1-phosphate and myo-inositol 1:2-cyclic monophosphate.

Citing Articles

Transcriptome analysis identifies genes involved in adventitious branches formation of Gracilaria lichenoides in vitro.

Wang W, Li H, Lin X, Yang S, Wang Z, Fang B Sci Rep. 2015; 5:17099.

PMID: 26657019 PMC: 4675990. DOI: 10.1038/srep17099.

Changes in the pattern of phospholipid synthesis during the induction by cytokinin of cell division in soybean suspension cultures.

Connett R, Hanke D Planta. 2013; 170(2):161-7.

PMID: 24232874 DOI: 10.1007/BF00397884.

Kinetin-induced stimulation of electrogenic pumping in soybean suspension cultures is unrelated to signal transduction.

Parsons A, Blackford S, Sanders D Planta. 2013; 178(2):215-22.

PMID: 24212751 DOI: 10.1007/BF00393197.

References

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

Irvine R . The enzymology of stimulated inositol lipid turnover. Cell Calcium. 1982; 3(4-5):295-309. DOI: 10.1016/0143-4160(82)90018-5. View

Lapetina E, Michell R . A membrane-bound activity catalysing phosphatidylinositol breakdown to 1,2-diacylglycerol, D-myoinositol 1:2-cyclic phosphate an D-myoinositol 1-phosphate. Properties and subcellular distribution in rat cerebral cortex. Biochem J. 1973; 131(3):433-42. PMC: 1177491. DOI: 10.1042/bj1310433. View

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

Irvine R, Dawson R . The distribution of calcium-dependent phosphatidylinositol-specific phosphodiesterase in rat brain. J Neurochem. 1978; 31(6):1427-34. DOI: 10.1111/j.1471-4159.1978.tb06568.x. View

Lauchli A . Radioassay for beta-emitters in biological materials using Cerenkov radiation. Int J Appl Radiat Isot. 1969; 20(4):265-70. DOI: 10.1016/0020-708x(69)90054-4. View

BARTLETT G . Phosphorus assay in column chromatography. J Biol Chem. 1959; 234(3):466-8. View

Scott T, Dawson R . Metabolism of phospholipids by spermatozoa and seminal plasma. Biochem J. 1968; 108(3):457-63. PMC: 1198831. DOI: 10.1042/bj1080457. View

Michell R . Stimulated inositol lipid metabolism: an introduction. Cell Calcium. 1982; 3(4-5):285-94. DOI: 10.1016/0143-4160(82)90017-3. View

10.

Dawson R . Studies on the enzymic hydrolysis of monophosphoinositide by phospholipase preparations from P. notatum and ox pancreas. Biochim Biophys Acta. 1959; 33(1):68-77. DOI: 10.1016/0006-3002(59)90499-8. View

11.

Irvine R, Letcher A, Dawson R . Phosphatidylinositol phosphodiesterase in higher plants. Biochem J. 1980; 192(1):279-83. PMC: 1162332. DOI: 10.1042/bj1920279. View

12.

Dawson R, Clarke N . D-myoinositol 1:2-cyclic phosphate 2-phosphohydrolase. Biochem J. 1972; 127(1):113-8. PMC: 1178565. DOI: 10.1042/bj1270113. View

13.

Shukla S . Minireview. Phosphatidylinositol specific phospholipases C. Life Sci. 1982; 30(16):1323-35. DOI: 10.1016/0024-3205(82)90016-9. View

14.

Sumida S, Mudd J . The structure and biosynthesis of phosphatidyl inositol in cauliflower inflorescence. Plant Physiol. 1970; 45(6):712-8. PMC: 396499. DOI: 10.1104/pp.45.6.712. View

15.

Hanes C, ISHERWOOD F . Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949; 164(4183):1107-12, illust. DOI: 10.1038/1641107a0. View

16.

SKIPSKI V, Good J, Barclay M, REGGIO R . Quantitative analysis of simple lipid classes by thin-layer chromatography. Biochim Biophys Acta. 1968; 152(1):10-9. DOI: 10.1016/0005-2760(68)90003-9. View