Neonatal Human Foreskin Keratinocytes Produce 1,25-dihydroxyvitamin D3

Overview

Journal Biochemistry

Specialty Biochemistry

Date 1986 Apr 8

PMID 2423114

Citations 44

Authors

D D Bikle

M K Nemanic

J O Whitney

P W Elias

Affiliations

Soon will be listed here.

Abstract

Primary cultures of neonatal human foreskin keratinocytes converted 25-hydroxyvitamin D in high yield to a metabolite with the chromatographic behavior of 1,25-dihydroxyvitamin D3. The identity of this metabolite as 1,25-dihydroxyvitamin D3 was confirmed both by its potency in displacing 1,25-dihydroxyvitamin D3 in the chick cytosol receptor assay and by mass spectral analysis. These results suggest that 1,25-dihydroxyvitamin D3 may be formed in the epidermis to regulate vitamin D production by the epidermis and to provide an alternative to 1,25-dihydroxyvitamin D3 production by the kidneys.

Citing Articles

Ligand-Independent Vitamin D Receptor Actions Essential for Keratinocyte Homeostasis in the Skin.

Kise S, Morita S, Sakaki T, Kimura H, Kinuya S, Yasuda K Int J Mol Sci. 2025; 26(1.

PMID: 39796272 PMC: 11720424. DOI: 10.3390/ijms26010422.

1,25-Dihydroxyvitamin D Suppresses UV-Induced Poly(ADP-Ribose) Levels in Primary Human Keratinocytes, as Detected by a Novel Whole-Cell ELISA.

De Silva W, Sequeira V, Yang C, Dixon K, Holland A, Mason R Int J Mol Sci. 2024; 25(11).

PMID: 38891771 PMC: 11171802. DOI: 10.3390/ijms25115583.

The Cysteine Protease Legumain Is Upregulated by Vitamin D and Is a Regulator of Vitamin D Metabolism in Mice.

Forbord K, Okla M, Lunde N, Bosnjak-Olsen T, Arnekleiv G, Hesselson D Cells. 2024; 13(1).

PMID: 38201240 PMC: 10778535. DOI: 10.3390/cells13010036.

Vitamin D and Cardiovascular Diseases: An Update.

Haider F, Ghafoor H, Hassan O, Farooqui K, Bel Khair A, Shoaib F Cureus. 2024; 15(11):e49734.

PMID: 38161941 PMC: 10757591. DOI: 10.7759/cureus.49734.

Vitamin A, D, E, and K as Matrix Metalloproteinase-2/9 Regulators That Affect Expression and Enzymatic Activity.

Vo H, Nguyen Y, Kim N, Lee H Int J Mol Sci. 2023; 24(23).

PMID: 38069361 PMC: 10707015. DOI: 10.3390/ijms242317038.