Biosynthesis of Osmoregulated Periplasmic Glucans in Escherichia Coli: the Phosphoethanolamine Transferase is Encoded by OpgE

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 Nov 15

PMID 24228245

Citations 12

Authors

Sebastien Bontemps-Gallo

Virginie Cogez

Catherine Robbe-Masselot

Kevin Quintard

Jacqueline Dondeyne

Edwige Madec

Jean-Marie Lacroix

Affiliations

Soon will be listed here.

Abstract

Osmoregulated periplasmic glucans (OPGs) are oligosaccharides found in the periplasm of many Gram-negative bacteria. Glucose is the sole constitutive sugar and this backbone may be substituted by various kinds of molecules depending on the species. In E. coli, OPG are substituted by phosphoglycerol and phosphoethanolamine derived from membrane phospholipids and by succinyl residues. In this study, we describe the isolation of the opgE gene encoding the phosphoethanolamine transferase by a screen previously used for the isolation of the opgB gene encoding the phosphoglycerol transferase. Both genes show structural and functional similarities without sequence similarity.

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