Alpha 2.0
Login Register
» Articles » PMID: 2422203

Specific and Common Antigenic Determinants of Candida Albicans Isolates Detected by Monoclonal Antibody

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1986 Feb 1
PMID 2422203
Citations 9
Authors
L Polonelli
G Morace
Affiliations
Soon will be listed here.
Abstract

We isolated a hybridoma cell line which produced monoclonal antibody to one determinant of an exoantigen of Candida albicans. The immunoglobulin G antibody product was characterized by using a Western blot technique and was used for a serological analysis of numerous homologous and heterologous yeast isolates. Based on specific immunologic determinants, C. albicans strains were identified and clustered into five groups. The monoclonal antibodies were effective reagents for identifying and serotyping our C. albicans isolates; they have potential application in the epidemiology of yeast infections.

Citing Articles

Candida identification: a journey from conventional to molecular methods in medical mycology.

Alam M, Alam Q, Jiman-Fatani A, Kamal M, Abuzenadah A, Chaudhary A World J Microbiol Biotechnol. 2014; 30(5):1437-51.

PMID: 24379160 DOI: 10.1007/s11274-013-1574-z.

Production, characterization, and epitope mapping of a monoclonal antibody against aspartic proteinase of Candida albicans.

Na B, Chung G, Song C Clin Diagn Lab Immunol. 1999; 6(3):429-33.

PMID: 10225850 PMC: 103737. DOI: 10.1128/CDLI.6.3.429-433.1999.

Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies.

Freydiere A, Buchaille L, Guinet R, Gille Y J Clin Microbiol. 1997; 35(4):877-80.

PMID: 9157146 PMC: 229694. DOI: 10.1128/jcm.35.4.877-880.1997.

Killer systems and pathogenic fungi.

Polonelli L, Morace G Eur J Epidemiol. 1988; 4(4):415-8.

PMID: 3203721 DOI: 10.1007/BF00146391.

Production and characterization of murine monoclonal antibodies to Histoplasma capsulatum yeast cell antigens.

Kamel S, Wheat L, Garten M, Bartlett M, Tansey M, Tewari R Infect Immun. 1989; 57(3):896-901.

PMID: 2917790 PMC: 313195. DOI: 10.1128/iai.57.3.896-901.1989.

References
1.
Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View
2.
Taguchi M, Tsukiji M, Tsuchiya T . Rapid identification of yeasts by serological methods: a combined serological and biological method. Sabouraudia. 1979; 17(3):185-91. DOI: 10.1080/00362177985380271. View
3.
Braun D, Pereira L, Norrild B, Roizman B . Application of denatured, electrophoretically separated, and immobilized lysates of herpes simplex virus-infected cells for detection of monoclonal antibodies and for studies of the properties of viral proteins. J Virol. 1983; 46(1):103-12. PMC: 255097. DOI: 10.1128/JVI.46.1.103-112.1983. View
4.
Hasenclever H, Mitchell W . Antigenic studies of Candida. I. Observation of two antigenic groups in Candida albicans. J Bacteriol. 1961; 82:570-3. PMC: 279209. DOI: 10.1128/jb.82.4.570-573.1961. View
5.
LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View