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Immunogold Localization of Acyl Carrier Protein in Plants and Escherichia Coli: Evidence for Membrane Association in Plants

Overview
Journal Planta
Specialty Biology
Date 2013 Nov 14
PMID 24221708
Citations 6
Authors
A R Slabas
C G Smith
Affiliations
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Abstract

Immunogold labelling was used to study the distribution of acyl carrier protein (ACP) in Escherichia coli and a variety of plant tissues. In E. coli, ACP is distributed throughout the cytoplasm, confirming the observation of S. Jackowski et al. (1985, J. Bacteriol., 162, 5-8_. In the mesocarp of Avocado (Persea americana) and maturing seeds of oil-seed rape (Brassica napus cv. Jet Neuf), over 95% of the ACP is localised to plastids. The protein is almost exclusively located in the chloroplasts of leaf material from oil-seed rape. Approximately 80% of the gold particles associated with the ACP were further localized to the thylakoid membrane of the chloroplast. Since acetyl-CoA carboxylase has been reported to be localized to the thylakoid membrane (C.G. Kannangara and C.J. Jensen, 1975, Eur. J. Biochem., 54, 25-30), these results are consistent with the view that the two sequential enzymes in fatty-acid synthesis are in close spacial proximity.

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References
1.
van den Bosch H, Williamson J, VAGELOS P . Localization of acyl carrier protein in Escherichia coli. Nature. 1970; 228(5269):338-41. DOI: 10.1038/228338a0. View
2.
Simoni R, Criddle R, Stumpf P . Fat metabolism in higher plants. XXXI. Purification and properties of plant and bacterial acyl carrier proteins. J Biol Chem. 1967; 242(4):573-81. View
3.
Jackowski S, Edwards H, Davis D, Rock C . Localization of acyl carrier protein in Escherichia coli. J Bacteriol. 1985; 162(1):5-8. PMC: 218944. DOI: 10.1128/jb.162.1.5-8.1985. View
4.
Kannangara C, Jensen C . Biotin carboxyl carrier protein in barley chloroplast membranes. Eur J Biochem. 1975; 54(1):25-30. DOI: 10.1111/j.1432-1033.1975.tb04109.x. View
5.
Hanks J, Tolbert N, Schubert K . Localization of enzymes of ureide biosynthesis in peroxisomes and microsomes of nodules. Plant Physiol. 1981; 68(1):65-9. PMC: 425890. DOI: 10.1104/pp.68.1.65. View