High Resolution Bands in Maize Chromosomes by G-banding Methods

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 14

PMID 24220906

Citations 4

Authors

K Kakeda

H Yamagata

M Ohno

K Fukui

Z Z Wei

E S Zhu

Affiliations

Soon will be listed here.

Abstract

It was demonstrated that G-bands are unequivocally present in plant chromosomes, in contrast to what had been formerly believed by plant cytologists. Maize chromosomes prepared by an enzymatic maceration method and treated with trypsin or SDS showed clear G-bands spreading along the chromosomes. The most critical point during the G-banding procedures was the post-fixation with glutaraldehyde solution. Banding patterns were processed by using the chromosome image analyzing system and a clearer image was obtained. Gbanding technique and the image manipulation method described here can be applied to many plant species, and would contribute new information in the field of plant cytology and genetics.

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References

Rattner J, Lin C . Radial loops and helical coils coexist in metaphase chromosomes. Cell. 1985; 42(1):291-6. DOI: 10.1016/s0092-8674(85)80124-0. View

Sumner A, Evans H, Buckland R . New technique for distinguishing between human chromosomes. Nat New Biol. 1971; 232(27):31-2. DOI: 10.1038/newbio232031a0. View

Burkholder G, Duczek L . The effect of chromosome banding techniques on the proteins of isolated chromosomes. Chromosoma. 1982; 87(4):425-35. DOI: 10.1007/BF00327183. View

Ohnuki Y . Structure of chromosomes. I. Morphological studies of the spiral structure of human somatic chromosomes. Chromosoma. 1968; 25(4):402-28. DOI: 10.1007/BF02327721. View

Anderson L, Stack S, Mitchell J . An investigation of the basis of a current hypothesis for the lack of G-banding in plant chromosomes. Exp Cell Res. 1982; 138(2):433-6. DOI: 10.1016/0014-4827(82)90193-8. View

Korenberg J, Rykowski M . Human genome organization: Alu, lines, and the molecular structure of metaphase chromosome bands. Cell. 1988; 53(3):391-400. DOI: 10.1016/0092-8674(88)90159-6. View

Bennett M, Heslop-Harrison J, Smith J, Ward J . DNA density in mitotic and meiotic metaphase chromosomes of plants and animals. J Cell Sci. 1983; 63:173-9. DOI: 10.1242/jcs.63.1.173. View

Schweizer D . Counterstain-enhanced chromosome banding. Hum Genet. 1981; 57(1):1-14. View

Holmquist G, Gray M, Porter T, Jordan J . Characterization of Giemsa dark- and light-band DNA. Cell. 1982; 31(1):121-9. DOI: 10.1016/0092-8674(82)90411-1. View

10.

Schweizer D . Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma. 1976; 58(4):307-24. DOI: 10.1007/BF00292840. View

11.

McAlpine P, Van Cong N, Boucheix C, Pakstis A, Doute R, Shows T . The 1987 Catalog of mapped genes and report of the nomenclature committee. Cytogenet Cell Genet. 1987; 46(1-4):29-101. DOI: 10.1159/000132472. View

12.

Greilhuber J . Why plant chromosomes do not show G-bands. Theor Appl Genet. 2014; 50(3):121-4. DOI: 10.1007/BF00276805. View

13.

Fukui K . Standardization of karyotyping plant chromosomes by a newly developed chromosome image analyzing system (CHIAS). Theor Appl Genet. 2013; 72(1):27-32. DOI: 10.1007/BF00261449. View

14.

Takayama S . Configurational changes in chromatids from helical to banded structures. Chromosoma. 1976; 56(1):47-54. DOI: 10.1007/BF00293729. View

15.

Goldman M, Holmquist G, Gray M, Caston L, Nag A . Replication timing of genes and middle repetitive sequences. Science. 1984; 224(4650):686-92. DOI: 10.1126/science.6719109. View

16.

JAGIELLO G, Fang J . A pachytene map of the mouse oocyte. Chromosoma. 1980; 77(1):113-21. DOI: 10.1007/BF00292045. View