Anti-oxidizing Effect of the Dichloromethane and Hexane Fractions from Orostachys Japonicus in LPS-stimulated RAW 264.7 Cells Via Upregulation of Nrf2 Expression and Activation of MAPK Signaling Pathway

Overview

Journal BMB Rep

Specialties Biochemistry
Molecular Biology

Date 2013 Nov 14

PMID 24219867

Citations 15

Authors

Hyeong-Seon Lee

Gyeong-Seon Lee

Seon-Hee Kim

Hyun-Kyung Kim

Dong-Hee Suk

Dong-Seok Lee

Affiliations

Soon will be listed here.

Abstract

Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway.

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