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Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells

Overview
Journal Cancers (Basel)
Publisher MDPI
Specialty Oncology
Date 2013 Nov 13
PMID 24216983
Citations 4
Authors
Affiliations
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Abstract

Cooperation between endothelial cells and bone in bone remodelling is well established. In contrast, bone microvasculature supporting the growth of primary tumors and metastasis is poorly understood. Several antiangiogenic agents have recently been undergoing trials, although an extensive body of clinical data and experimental research have proved that angiogenic pathways differ in each tumor type and stage. Here, for the first time, we characterize at the molecular and functional level tumor endothelial cells from human bone sarcomas at different stages of disease and with different histotypes. We selected a CD31+ subpopulation from biopsies that displayed the capability to grow as adherent cell lines without vascular endothelial growth factor (VEGF). Our findings show the existence in human primary bone sarcomas of highly proliferative endothelial cells expressing CD31, CD44, CD105, CD146 and CD90 markers. These cells are committed to develop capillary-like structures and colony formation units, and to produce nitric oxide. We believe that a better understanding of tumor vasculature could be a valid tool for the design of an efficacious antiangiogenic therapy as adjuvant treatment of sarcomas.

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References
1.
St Croix B, Rago C, Velculescu V, Traverso G, Romans K, Montgomery E . Genes expressed in human tumor endothelium. Science. 2000; 289(5482):1197-202. DOI: 10.1126/science.289.5482.1197. View

2.
Regan E, Aird W . Dynamical systems approach to endothelial heterogeneity. Circ Res. 2012; 111(1):110-30. PMC: 3400545. DOI: 10.1161/CIRCRESAHA.111.261701. View

3.
Tomaso E, Capen D, Haskell A, Hart J, Logie J, Jain R . Mosaic tumor vessels: cellular basis and ultrastructure of focal regions lacking endothelial cell markers. Cancer Res. 2005; 65(13):5740-9. DOI: 10.1158/0008-5472.CAN-04-4552. View

4.
Zhu H, Shao Q, Sun X, Deng Z, Yuan X, Yu D . The mobilization, recruitment and contribution of bone marrow-derived endothelial progenitor cells to the tumor neovascularization occur at an early stage and throughout the entire process of hepatocellular carcinoma growth. Oncol Rep. 2012; 28(4):1217-24. DOI: 10.3892/or.2012.1944. View

5.
McDonald D, Foss A . Endothelial cells of tumor vessels: abnormal but not absent. Cancer Metastasis Rev. 2001; 19(1-2):109-20. DOI: 10.1023/a:1026529222845. View