Alpha 2.0
Login Register
» Articles » PMID: 24213348

The Use of IAA to Overcome Interspecific Hybrid Inviability in Reciprocal Crosses Between Nicotiana Tabacum L. and N. Repanda Willd

Overview
Journal Theor Appl Genet
Publisher Springer
Specialty Genetics
Date 2013 Nov 12
PMID 24213348
Citations 5
Authors
W M Zhou
K Yoshida
Y Shintaku
G Takeda
Affiliations
Soon will be listed here.
Abstract

Flowering hybrid plants were obtained from reciprocal crosses between N. tabacum L. (2n=48) and N. repanda Willd. (2n = 48), in which cross incompatibility and hybrid inviability were manifested. Stylar pollination and ovule culture were used to overcome the cross incompatibility of stylar barriers and ovular death. It was shown that application of 2 mg/l indole-3-acetic acid (IAA) during the growth period, from the fiveto six-leaf stage to the flowering stage, is a useful and easy method to overcome hybrid inviability.

Citing Articles

Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow.

He H, Shiragaki K, Tezuka T Front Plant Sci. 2023; 14:1219417.

PMID: 37476165 PMC: 10354522. DOI: 10.3389/fpls.2023.1219417.

Transcriptome and plant hormone analyses provide new insight into the molecular regulatory networks underlying hybrid lethality in cabbage (Brassica oleracea).

Xiao Z, Liu X, Fang Z, Yang L, Zhang Y, Wang Y Planta. 2021; 253(5):96.

PMID: 33839925 DOI: 10.1007/s00425-021-03608-1.

Interspecific hybrid between Allium cepa and Allium sativum.

Ohsumi C, Kojima A, Hinata K, Etoh T, Hayashi T Theor Appl Genet. 2013; 85(8):969-75.

PMID: 24196147 DOI: 10.1007/BF00215036.

Cell death in seedlings of the interspecific hybrid of Nicotiana gossei and N. tabacum; possible role of knob-like bodies formed on tonoplast in vacuolar-collapse-mediated cell death.

Mino M, Murata N, Date S, Inoue M Plant Cell Rep. 2007; 26(4):407-19.

PMID: 17356884 DOI: 10.1007/s00299-006-0261-z.

Hybrid lethality of cultured cells of an interspecific F1 hybrid of Nicotiana gossei Domin and N. tabacum L.

Mino M, Misaka Y, Ueda J, Ogawa K, Inoue M Plant Cell Rep. 2005; 24(3):179-88.

PMID: 15714321 DOI: 10.1007/s00299-005-0923-2.

References
1.
Nitsch J, Nitsch C . Haploid plants from pollen grains. Science. 1969; 163(3862):85-7. DOI: 10.1126/science.163.3862.85. View
2.
Shintaku Y, Yamamoto K, Nakajima T . Interspecific hybridization between Nicotiana repanda Willd. and N. tabacum L. through the pollen irradiation technique and the egg cell irradiation technique. Theor Appl Genet. 2013; 76(2):293-8. DOI: 10.1007/BF00257858. View
3.
Stavely J, Pittarelli G, Burk L . Nicotiana repanda as a potential source for disease resistance in N. tabacum. J Hered. 1973; 64(5):265-71. DOI: 10.1093/oxfordjournals.jhered.a108409. View
4.
Deverna J, Myers J, Collins G . Bypassing prefertilization barriers to hybridization in Nicotiana using in vitro pollination and fertilization. Theor Appl Genet. 2013; 73(5):665-71. DOI: 10.1007/BF00260773. View
5.
Holm R, Abeles F . The role of ethylene in 2.4-D-induced growth inhibition. Planta. 2014; 78(3):293-304. DOI: 10.1007/BF00386430. View