Complex Determination of Male Sterility in Thymus Vulgaris L.: Genetic and Molecular Analysis

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 12

PMID 24213057

Citations 9

Authors

E Belhassen

B Dommee

A Atlan

P H Gouyon

D Pomente

M W Assouad

D Couvet

Affiliations

Soon will be listed here.

Abstract

Nucleocytoplasmic determination of male sterility in Thymus vulgaris L. has been assumed in all papers attempting to explain the remarkably high frequencies of male steriles found in natural populations of this species. This paper provides strong evidence that both nuclear and cytoplasmic genes are involved in the determination of male sterility of this species, giving a complex inheritance. Interpopulation and intrapopulation crosses have shown that the ratio of females versus hermaphrodites among offsprings varied widely from 1∶0 to 1∶1. Furthermore, interpopulation crosses consistently yielded a higher frequency of females than intrapopulation crosses. Nucleocytoplasmic inheritance was demonstrated by an absence of male fertiles in backcrosses and asymmetrical segregation in reciprocal crosses. Molecular analysis of the mitochondrial DNA of some of the parents used in crosses suggested the involvement of different cytoplasms in the inheritance of male steriliy.

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References

Birky Jr C . Transmission genetics of mitochondria and chloroplasts. Annu Rev Genet. 1978; 12:471-512. DOI: 10.1146/annurev.ge.12.120178.002351. View

Boutin V, Pannenbecker G, Ecke W, Schewe G, Saumitou-Laprade P, Jean R . Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects. Theor Appl Genet. 2013; 73(5):625-9. DOI: 10.1007/BF00260768. View

Cosmides L, Tooby J . Cytoplasmic inheritance and intragenomic conflict. J Theor Biol. 1981; 89(1):83-129. DOI: 10.1016/0022-5193(81)90181-8. View

Thiellement H . Cytoplasmic male sterility in Vicia faba L. : Part 6: Genetical arguments for cytoplasmic heterogeneity. Theor Appl Genet. 2013; 61(1):47-52. DOI: 10.1007/BF00261509. View

Belhassen E, Trabaud L, Couvet D, Gouyon P . AN EXAMPLE OF NONEQUILIBRIUM PROCESSES: GYNODIOECY OF THYMUS VULGARIS L. IN BURNED HABITATS. Evolution. 2017; 43(3):662-667. DOI: 10.1111/j.1558-5646.1989.tb04262.x. View

Forde B, Leaver C . Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc Natl Acad Sci U S A. 1980; 77(1):418-22. PMC: 348282. DOI: 10.1073/pnas.77.1.418. View

Dewey R, Timothy D, Levings C . A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize. Proc Natl Acad Sci U S A. 1987; 84(15):5374-8. PMC: 298858. DOI: 10.1073/pnas.84.15.5374. View

Boutry M, Faber A, Charbonnier M, Briquet M . Microanalysis of plant mitochondrial protein synthesis products: : Detection of variant polypeptides associated with cytoplasmic male sterility. Plant Mol Biol. 2013; 3(6):445-52. DOI: 10.1007/BF00033392. View

Delannay X, Gouyon P, Valdeyron G . Mathematical Study of the Evolution of Gynodioecy with Cytoplasmic Inheritance under the Effect of a Nuclear Restorer Gene. Genetics. 1981; 99(1):169-81. PMC: 1214488. View

10.

Dixon L, Leaver C . Mitochondrial gene expression and cytoplasmic male sterility in sorghum. Plant Mol Biol. 2013; 1(2):89-102. DOI: 10.1007/BF00024973. View

11.

Bailey-Serres J, Dixon L, Liddell A, Leaver C . Nuclear-mitochondrial interactions in cytoplasmic male-sterile sorghum. Theor Appl Genet. 2013; 73(2):252-60. DOI: 10.1007/BF00289282. View

12.

Falconet D, Lejeune B, Quetier F, Gray M . Evidence for homologous recombination between repeated sequences containing 18S and 5S ribosomal RNA genes in wheat mitochondrial DNA. EMBO J. 1984; 3(2):297-302. PMC: 557337. DOI: 10.1002/j.1460-2075.1984.tb01799.x. View

13.

Lebacq P, Squalli D, Duchenne M, Pouletty P, Joannes M . A new sensitive non-isotopic method using sulfonated probes to detect picogram quantities of specific DNA sequences on blot hybridization. J Biochem Biophys Methods. 1988; 15(5):255-66. DOI: 10.1016/0165-022x(88)90013-9. View

14.

Bonen L, Boer P, Gray M . The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. EMBO J. 1984; 3(11):2531-6. PMC: 557724. DOI: 10.1002/j.1460-2075.1984.tb02168.x. View

15.

Singh A, Laughnan J . Instability of s male-sterile cytoplasm in maize. Genetics. 1972; 71(4):607-20. PMC: 1212796. DOI: 10.1093/genetics/71.4.607. View