Effects of Lysophosphatidylcholine on Resting Potassium Conductance of Isolated Guinea Pig Ventricular Cells

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1986 Mar 1

PMID 2421244

Citations 19

Authors

T Kiyosue

M Arita

Affiliations

Soon will be listed here.

Abstract

We studied the effects of lysophosphatidylcholine (LPC), a toxic metabolite of ischemia, on the inward rectifier potassium channel current in isolated guinea pig ventricular cells. LPC (10-50 microM) added to the external solution decreased the resting membrane potential and occasionally induced repetitive action potential discharges, with or without loss of repolarization. In voltage clamp studies, LPC (20 microM) decreased the conductance at the levels of resting potentials (approximately equal to -80 mV) from 26 +/- 8 nS to 16 +/- 3 nS (mean and SD, n = 4) within 10 min. Prolonged application of LPC (greater than 12 min) produced transient inward currents after depolarizing clamp pulses, thereby suggesting that the LPC elevated intracellular Ca2+ concentrations. The effect of LPC on the single inward rectifier K channel current was examined using the patch clamp technique in a cell-attached mode. LPC decreased the single channel conductance, depending on the concentration (5-100 microM). The slope conductance in the presence of 150 mM K+ in the pipette decreased from 45 +/- 7 pS (control) to 32 +/- 17, 20 +/- 19, and 14 +/- 10 pS for 5, 20 and 100 microM LPC, respectively. LPC induced little change with regard to probability of the channel opening. These results suggest that LPC depolarizes membrane by decreasing single channel conductance of the inward rectifier K channel. This reduction partially contributes to the alleged LPC-induced abnormal automaticities and conduction disturbances in the heart.

Citing Articles

Differential effects of lysophospholipids on exocytosis in rat PC12 cells.

Ma M, Yeo J, Farooqui A, Zhang J, Chen P, Ong W J Neural Transm (Vienna). 2010; 117(3):301-8.

PMID: 20058038 DOI: 10.1007/s00702-009-0355-1.

Involvement of A pertussis Toxin Sensitive G-Protein in the Inhibition of Inwardly Rectifying K Currents by Platelet-Activating Factor in Guinea-Pig Atrial Cardiomyocytes.

Gollasch M, Kleppisch T, Krautwurst D, Lewinsohn D, Hescheler J Mediators Inflamm. 1994; 3(1):45-51.

PMID: 18472923 PMC: 2367020. DOI: 10.1155/S0962935194000086.

Agonist potency at P2X7 receptors is modulated by structurally diverse lipids.

Michel A, Fonfria E Br J Pharmacol. 2007; 152(4):523-37.

PMID: 17700717 PMC: 2050815. DOI: 10.1038/sj.bjp.0707417.

Cell membrane-derived lysophosphatidylcholine activates cardiac ryanodine receptor channels.

Nakamura Y, Yasukochi M, Kobayashi S, Uehara K, Honda A, Inoue R Pflugers Arch. 2006; 453(4):455-62.

PMID: 17028837 DOI: 10.1007/s00424-006-0141-y.

Physiological mechanisms of lysophosphatidylcholine-induced de-ramification of murine microglia.

Schilling T, Lehmann F, Ruckert B, Eder C J Physiol. 2004; 557(Pt 1):105-20.

PMID: 15020687 PMC: 1665039. DOI: 10.1113/jphysiol.2004.060632.

References

Josephson I, Brown A . A comparison of calcium currents in rat and guinea pig single ventricular cells. Circ Res. 1984; 54(2):144-56. DOI: 10.1161/01.res.54.2.144. View

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Taniguchi J, Kokubun S, Noma A, Irisawa H . Spontaneously active cells isolated from the sino-atrial and atrio-ventricular nodes of the rabbit heart. Jpn J Physiol. 1981; 31(4):547-58. DOI: 10.2170/jjphysiol.31.547. View

Meier Jr C, KATZUNG B . Cesium blockade of delayed outward currents and electrically induced pacemaker activity in mammalian ventricular myocardium. J Gen Physiol. 1981; 77(5):531-47. PMC: 2215440. DOI: 10.1085/jgp.77.5.531. View

Kass R, Lederer W, Tsien R, Weingart R . Role of calcium ions in transient inward currents and aftercontractions induced by strophanthidin in cardiac Purkinje fibres. J Physiol. 1978; 281:187-208. PMC: 1282691. DOI: 10.1113/jphysiol.1978.sp012416. View

Mascher D . Electrical and mechanical responses in ventricular muscle fibers during barium perfusion. Pflugers Arch. 1973; 342(4):325-46. DOI: 10.1007/BF00586104. View

Sedlis S, Corr P, Sobel B, Ahumada G . Lysophosphatidyl choline potentiates Ca2+ accumulation in rat cardiac myocytes. Am J Physiol. 1983; 244(1):H32-8. DOI: 10.1152/ajpheart.1983.244.1.H32. View

Isenberg G, Klockner U . Calcium tolerant ventricular myocytes prepared by preincubation in a "KB medium". Pflugers Arch. 1982; 395(1):6-18. DOI: 10.1007/BF00584963. View

Morena H, Janse M, Fiolet J, Krieger W, Crijns H, Durrer D . Comparison of the effects of regional ischemia, hypoxia, hyperkalemia, and acidosis on intracellular and extracellular potentials and metabolism in the isolated porcine heart. Circ Res. 1980; 46(5):634-46. DOI: 10.1161/01.res.46.5.634. View

10.

Sakmann B, Trube G . Conductance properties of single inwardly rectifying potassium channels in ventricular cells from guinea-pig heart. J Physiol. 1984; 347:641-57. PMC: 1199469. DOI: 10.1113/jphysiol.1984.sp015088. View

11.

Corr P, Cain M, Witkowski F, Price D, Sobel B . Potential arrhythmogenic electrophysiological derangements in canine Purkinje fibers induced by lysophosphoglycerides. Circ Res. 1979; 44(6):822-32. DOI: 10.1161/01.res.44.6.822. View

12.

Clarkson C, Ten Eick R . On the mechanism of lysophosphatidylcholine-induced depolarization of cat ventricular myocardium. Circ Res. 1983; 52(5):543-56. DOI: 10.1161/01.res.52.5.543. View

13.

Downar E, Janse M, Durrer D . The effect of "ischemic" blood on transmembrane potentials of normal porcine ventricular myocardium. Circulation. 1977; 55(3):455-62. DOI: 10.1161/01.cir.55.3.455. View

14.

Arnsdorf M, Sawicki G . The effects of lysophosphatidylcholine, a toxic metabolite of ischemia, on the components of cardiac excitability in sheep Purkinje fibers. Circ Res. 1981; 49(1):16-30. DOI: 10.1161/01.res.49.1.16. View

15.

Corr P, Gross R, Sobel B . Arrhythmogenic amphiphilic lipids and the myocardial cell membrane. J Mol Cell Cardiol. 1982; 14(11):619-26. DOI: 10.1016/0022-2828(82)90159-6. View

16.

Neher E, Steinbach J . Local anaesthetics transiently block currents through single acetylcholine-receptor channels. J Physiol. 1978; 277:153-76. PMC: 1282384. DOI: 10.1113/jphysiol.1978.sp012267. View

17.

Kiyosue T, Aomine M, Arita M . Lysophosphatidylcholine decreases single channel conductance of inward rectifier K channel in mammalian ventricular myocytes. Jpn J Physiol. 1984; 34(2):369-73. DOI: 10.2170/jjphysiol.34.369. View

18.

Kleber A, Janse M, van Capelle F, Durrer D . Mechanism and time course of S-T and T-Q segment changes during acute regional myocardial ischemia in the pig heart determined by extracellular and intracellular recordings. Circ Res. 1978; 42(5):603-13. DOI: 10.1161/01.res.42.5.603. View

19.

Noma A, Nakayama T, Kurachi Y, Irisawa H . Resting K conductances in pacemaker and non-pacemaker heart cells of the rabbit. Jpn J Physiol. 1984; 34(2):245-54. DOI: 10.2170/jjphysiol.34.245. View

20.

Matsuda H . Effects of intracellular calcium injection on steady state membrane currents in isolated single ventricular cells. Pflugers Arch. 1983; 397(1):81-3. DOI: 10.1007/BF00585176. View