» Articles » PMID: 24211391

JNK1 and 2 Play a Negative Role in Reprogramming to Pluripotent Stem Cells by Suppressing Klf4 Activity

Overview
Journal Stem Cell Res
Publisher Elsevier
Specialty Cell Biology
Date 2013 Nov 12
PMID 24211391
Citations 16
Authors
Affiliations
Soon will be listed here.
Abstract

Embryonic stem (ES) cells are pluripotent cells with the capacity for unlimited self-renewal or differentiation. Inhibition of MAPK pathways enhances mouse ES cell pluripotency characteristics. Compared to wildtype ES cells, jnk2(-/-) ES cells displayed a much higher growth rate. To determine whether JNKs are required for stem cell self-renewal or differentiation, we performed a phosphorylation kinase array assay to compare mouse ES cells under LIF+ or LIF- culture conditions. The data showed that activation of JNKs was induced by LIF withdrawal. We also found that JNK1 or 2 phosphorylated Klf4 at threonines 224 and 225. Activation of JNK signaling and phosphorylation of Klf4 inhibited Klf4 transcription and transactivation activity. Importantly, jnk1(-/-) and jnk2(-/-) murine embryonic fibroblasts (MEFs) exhibited a significantly greater potency in the ability to increase the number of iPS colonies compared with jnk wildtype MEFs. Overall, our results demonstrated that JNK1 and 2 play a negative role in reprogramming to pluripotent stem cells by suppressing Klf4 activity.

Citing Articles

Experimental study on small molecule combinations inducing reprogramming of rat fibroblasts into functional neurons.

Gao Q, Dai Z, Yang X, Liu C, Liu G Zhejiang Da Xue Xue Bao Yi Xue Ban. 2024; 53(4):498-508.

PMID: 39183062 PMC: 11375488. DOI: 10.3724/zdxbyxb-2024-0007.


Metabolic control of induced pluripotency.

Sinenko S, Tomilin A Front Cell Dev Biol. 2024; 11:1328522.

PMID: 38274274 PMC: 10808704. DOI: 10.3389/fcell.2023.1328522.


Characteristics of SP600125 Induced Tetraploid Cells in Comparison With Diploid and Tetraploid Cells of Fish.

Fan Y, Zhang G, Zhao K, Fu W, Chen S, Liu J Front Genet. 2021; 12:781007.

PMID: 34938322 PMC: 8685524. DOI: 10.3389/fgene.2021.781007.


MAP2K6 remodels chromatin and facilitates reprogramming by activating Gatad2b-phosphorylation dependent heterochromatin loosening.

Xing G, Liu Z, Huang L, Zhao D, Wang T, Yuan H Cell Death Differ. 2021; 29(5):1042-1054.

PMID: 34815549 PMC: 9090911. DOI: 10.1038/s41418-021-00902-z.


circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.

Yan J, Yang Y, Fan X, Liang G, Wang Z, Li J J Cachexia Sarcopenia Muscle. 2021; 13(1):696-712.

PMID: 34811940 PMC: 8818660. DOI: 10.1002/jcsm.12859.