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Somatic Hybridization Between Brassica Juncea and Moricandia Arvensis by Protoplast Fusion

Overview
Journal Plant Cell Rep
Publisher Springer
Date 2013 Nov 9
PMID 24203148
Citations 12
Authors
P B Kirti
S B Narasimhulu
S Prakash
V L Chopra
Affiliations
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Abstract

Intergeneric somatic hybrids have been produced between Brassica juncea (2n=36, AABB) cv. RLM-198 and Moricandia arvensis (2n=28, MM) by protoplast fusion. Hypocotyl protoplasts of B. juncea were fused with mesophyll protoplasts of M. arvensis using polyethylene glycol. Fusion frequency, estimated on the basis of differential morphological characterstics of parental protoplasts was about 5%. Of the 156 calli obtained, four calli produced shoots intermediate in morphology between the parents. Hybrid nature of the plants was confirmed using wheat nuclear rDNA probe. Hybridization of total DNA with a mitochondrial DNA probe carrying 5s-18s rRNA genes of maize showed that the mitochondria of the somatic hybrids were derived from the wild species M. arvensis. Meiosis in the only hybrid that produced normal flowers revealed the occurrence of 64 chromosomes, the sum of chromosomes of parental species. Inspite of complete pollen sterility, siliquas were produced in this hybrid by back-crossing with B. juncea. These siliquas on in vitro culture produced 12 seeds.

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References
1.
Kirti P, Narasimhulu S, Prakash S, Chopra V . Production and characterization of intergeneric somatic hybrids of Trachystoma ballii and Brassica juncea. Plant Cell Rep. 2013; 11(2):90-2. DOI: 10.1007/BF00235260. View
2.
Chao S, Sederoff R, Levings C . Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of the Maize Mitochondrial Genome. Plant Physiol. 1983; 71(1):190-3. PMC: 1067201. DOI: 10.1104/pp.71.1.190. View
3.
Takahata Y, Takeda T . Intergeneric (intersubtribe) hybridization between Moricandia arvensis and Brassica A and B genome species by ovary culture. Theor Appl Genet. 2013; 80(1):38-42. DOI: 10.1007/BF00224013. View
4.
Robertson D, Palmer J, Earle E, Mutschler M . Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris. Theor Appl Genet. 2013; 74(3):303-9. DOI: 10.1007/BF00274711. View
5.
Rosen B, Hallden C, Heneen W . Diploid Brassica napus somatic hybrids: characterization of nuclear and organellar DNA. Theor Appl Genet. 2013; 76(2):197-203. DOI: 10.1007/BF00257846. View