Transformation of Rapid Cycling Cabbage (Brassica Oleracea Var. Capitata) with Agrobacterium Rhizogenes

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2013 Nov 9

PMID 24201433

Citations 4

Authors

P Berthomieu

L Jouanin

Affiliations

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Abstract

Genetically transformed cabbage (Brassica oleracea var. capitata) roots were obtained after inoculation with two engineered Agrobacterium rhizogenes strains, each harbouring a plant selectable marker gene in their T-DNA. Axenic root clones resistant to kanamycin or hygromycin B were established, most of which did not exhibit the phenotypic characteristics of Ri-transformed roots. Shoot regeneration was induced from roots after treatment with 2,4-dichlorophenoxyacetic acid (2,4-D). The resulting plants exhibited various phenotypes: some looked normal, while others showed the transformed phenotype observed in other species. Direct evidence for genetic transformation was obtained by molecular hybridization. The trait was transmitted to the progeny. Transformed cabbage plants can be obtained within 6 months using this approach.

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