In Vivo Splicing of the PremRNAs from Early Region 3 of Adenovirus-2: the Products of Cleavage at the 5' Splice Site of the Common Intron

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Feb 11

PMID 2419834

Citations 10

Authors

A Sittler

H Gallinaro

M Jacob

Affiliations

Soon will be listed here.

Abstract

The nuclear transcripts of the early region 3 from adenovirus-2 were studied for the presence of the cleavage products of premRNA at the 5' splice site of the first intervening sequence. Two molecules, free exon 1 and intron-exon 2-poly(A) were characterized by complementary methods including Northern blotting, RNase and S1 nuclease mapping, hybrid-selection and primer extension. The intron of the intron-exon 2 molecule is at least partially in a lariat form. A study of the time course of appearance of the 2 molecules shows that their formation occurs after the synthesis of premRNA, the lag period being no more than 1-2 min and before the formation of mature mRNA which starts accumulating after 3-4 min. These data are compatible with the idea that the 5' cleavage products are splicing intermediates in vivo.

Citing Articles

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Upstream and downstream cis-acting elements for cleavage at the L4 polyadenylation site of adenovirus-2.

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Genetic analysis of mRNA synthesis in adenovirus region E3 at different stages of productive infection by RNA-processing mutants.

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The different intron 2 species excised in vivo from the E2A premRNA of adenovirus-2: an approach to analyse alternative splicing.

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