Testing the Anti-fibrotic Potential of the Single-chain Fv Antibody Against the α2 C-terminal Telopeptide of Collagen I

Overview

Journal Connect Tissue Res

Publisher Informa Healthcare

Date 2013 Nov 8

PMID 24195607

Citations 5

Authors

Jolanta Fertala

James Kostas

Cheryl Hou

Andrzej Steplewski

Pedro Beredjiklian

Joseph Abboud

William V Arnold

Gerald Williams

Andrzej Fertala

Affiliations

Soon will be listed here.

Abstract

Abstract This study focuses on the single-chain fragment variable (scFv) variant of the original IgA-type antibody, recognizing the α2 C-terminal telopeptide (α2Ct) of human collagen I, designed to inhibit post-traumatic localized fibrosis via blocking the formation of collagen-rich deposits. We have demonstrated that the scFv construct expressed in yeast cells was able to fold into an immunoglobulin-like conformation, but it was prone to forming soluble aggregates. Functional assays, however, indicate that the scFv construct specifically binds to the α2Ct epitope and inhibits collagen fibril formation both in vitro and in a cell culture model representing tissues that undergo post-traumatic fibrosis. Thus, the presented study demonstrates the potential of the scFv variant to serve as an inhibitor of the excessive formation of collagen-rich fibrotic deposits, and it reveals certain limitations associated with the current stage of development of this antibody construct.

Citing Articles

Extracellular Targets to Reduce Excessive Scarring in Response to Tissue Injury.

Fertala J, Wang M, Rivlin M, Beredjiklian P, Abboud J, Arnold W Biomolecules. 2023; 13(5).

PMID: 37238628 PMC: 10216402. DOI: 10.3390/biom13050758.

Mechanisms of reducing joint stiffness by blocking collagen fibrillogenesis in a rabbit model of posttraumatic arthrofibrosis.

Steplewski A, Fertala J, Tomlinson R, Wang M, Donahue A, Arnold W PLoS One. 2021; 16(9):e0257147.

PMID: 34492074 PMC: 8423260. DOI: 10.1371/journal.pone.0257147.

Collagen Structure-Function Mapping Informs Applications for Regenerative Medicine.

San Antonio J, Jacenko O, Fertala A, Orgel J Bioengineering (Basel). 2021; 8(1).

PMID: 33383610 PMC: 7824244. DOI: 10.3390/bioengineering8010003.

Target-Specific Delivery of an Antibody That Blocks the Formation of Collagen Deposits in Skin and Lung.

Fertala J, Romero F, Summer R, Fertala A Monoclon Antib Immunodiagn Immunother. 2017; 36(5):199-207.

PMID: 28972447 PMC: 5655468. DOI: 10.1089/mab.2017.0044.

Matrix-specific anchors: a new concept for targeted delivery and retention of therapeutic cells.

Steplewski A, Fertala J, Beredjiklian P, Wang M, Fertala A Tissue Eng Part A. 2014; 21(7-8):1207-16.

PMID: 25435302 PMC: 4394883. DOI: 10.1089/ten.tea.2014.0401.

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