Metabolic Flux and Compartmentation Analysis in the Brain In Vivo

Overview

Journal Front Endocrinol (Lausanne)

Specialty Endocrinology

Date 2013 Nov 7

PMID 24194729

Citations 27

Authors

Bernard Lanz

Rolf Gruetter

Joao M N Duarte

Affiliations

Soon will be listed here.

Abstract

Through significant developments and progresses in the last two decades, in vivo localized nuclear magnetic resonance spectroscopy (MRS) became a method of choice to probe brain metabolic pathways in a non-invasive way. Beside the measurement of the total concentration of more than 20 metabolites, (1)H MRS can be used to quantify the dynamics of substrate transport across the blood-brain barrier by varying the plasma substrate level. On the other hand, (13)C MRS with the infusion of (13)C-enriched substrates enables the characterization of brain oxidative metabolism and neurotransmission by incorporation of (13)C in the different carbon positions of amino acid neurotransmitters. The quantitative determination of the biochemical reactions involved in these processes requires the use of appropriate metabolic models, whose level of details is strongly related to the amount of data accessible with in vivo MRS. In the present work, we present the different steps involved in the elaboration of a mathematical model of a given brain metabolic process and its application to the experimental data in order to extract quantitative brain metabolic rates. We review the recent advances in the localized measurement of brain glucose transport and compartmentalized brain energy metabolism, and how these reveal mechanistic details on glial support to glutamatergic and GABAergic neurons.

Citing Articles

Challenges of Investigating Compartmentalized Brain Energy Metabolism Using Nuclear Magnetic Resonance Spectroscopy in vivo.

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Mapping of glutamate metabolism using 1H FID-MRSI after oral administration of [1-13C]Glc at 9.4 T.

Ziegs T, Ruhm L, Wright A, Henning A Neuroimage. 2023; 270:119940.

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C metabolic flux analysis: Classification and characterization from the perspective of mathematical modeling and application in physiological research of neural cell.

Tian B, Chen M, Liu L, Rui B, Deng Z, Zhang Z Front Mol Neurosci. 2022; 15:883466.

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Measurement of glucose metabolism in the occipital lobe and frontal cortex after oral administration of [1-13C]glucose at 9.4 T.

Ziegs T, Dorst J, Ruhm L, Avdievitch N, Henning A J Cereb Blood Flow Metab. 2022; 42(10):1890-1904.

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Measuring Glycolytic Activity with Hyperpolarized [H, U-C] D-Glucose in the Naive Mouse Brain under Different Anesthetic Conditions.

Flatt E, Lanz B, Pilloud Y, Capozzi A, Lerche M, Gruetter R Metabolites. 2021; 11(7).

PMID: 34201777 PMC: 8303162. DOI: 10.3390/metabo11070413.

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