Plasma Amino Acids Are Highly Dependent on Acid-base Balance in Uremic Rats

Overview

Journal Amino Acids

Specialty Biochemistry

Date 2013 Nov 7

PMID 24193029

Authors

N Gretz

K Langer

M Strauch

Affiliations

Soon will be listed here.

Abstract

Acidosis is a common finding in uremia. We analysed the effect of changes in acid-base balance in 42 Sprague-Dawley rats having longstanding uremia (> 3 mon). The rats were treated with CaCO3 or CaCl2 in a random cross-over trial after being divided into two dietary groups (8% or 18% protein). At the end of each observation period parameters were assessed. The main finding was that plasma amino and keto acid concentrations varied significantly with the acid-base balance and the protein intake. Furthermore a considerable degree of catabolism and anorexia was observed. In addition other confounding variables were observed, e.g. increased corticosterone excretion, electrolyte losses, and proteinuria. Our data underline that plasma amino acid concentrations in uremic rats are highly dependent on acid-base balance and overall protein intake.

References

Brenner B, Meyer T, Hostetter T . Dietary protein intake and the progressive nature of kidney disease: the role of hemodynamically mediated glomerular injury in the pathogenesis of progressive glomerular sclerosis in aging, renal ablation, and intrinsic renal disease. N Engl J Med. 1982; 307(11):652-9. DOI: 10.1056/NEJM198209093071104. View

Kleinknecht C, Gros F, Jean G, Broyer M . Growth and free amino acids in uremic hyperphagic rats. Am J Clin Nutr. 1980; 33(7):1690-5. DOI: 10.1093/ajcn/33.7.1690. View

Salusky I, Jones M, Kopple J . Protein and amino acid metabolism in posterior hemicorpus of acutely uremic rats. Am J Physiol. 1983; 244(6):E615-23. DOI: 10.1152/ajpendo.1983.244.6.E615. View

Hara Y, MAY R, Kelly R, Mitch W . Acidosis, not azotemia, stimulates branched-chain, amino acid catabolism in uremic rats. Kidney Int. 1987; 32(6):808-14. DOI: 10.1038/ki.1987.280. View

Tackman J, TEWS J, HARPER A . Dietary disproportions of amino acids in the rat: effects on food intake, plasma and brain amino acids and brain serotonin. J Nutr. 1990; 120(5):521-33. DOI: 10.1093/jn/120.5.521. View

Mannan A, Wiebe L, Noujaim A, Secord D . Plasma free amino acids in the chronically uremic rat. Clin Biochem. 1975; 8(5):303-6. DOI: 10.1016/s0009-9120(75)93350-0. View

Brenner B, Bohrer M, Baylis C, Deen W . Determinants of glomerular permselectivity: Insights derived from observations in vivo. Kidney Int. 1977; 12(4):229-37. DOI: 10.1038/ki.1977.107. View

BRODSKY W, Nelson N, GUEST G . Influence of food intake on ketosis, mineral balance and survival of alloxan-diabetic rats. Metabolism. 1952; 1(1):68-79. View

Wang M, Vyhmeister I, Kopple J, SWENDSEID M . Effect of protein intake on weight gain and plasma amino acid levels in uremic rats. Am J Physiol. 1976; 230(5):1455-9. DOI: 10.1152/ajplegacy.1976.230.5.1455. View

10.

Kihlberg R, Sterner G, Wennberg A, DENNEBERG T . Plasma free amino acid levels in uremic rats given high and low protein diets or intravenous infusions of amino acid solutions. J Nutr. 1982; 112(11):2058-70. DOI: 10.1093/jn/112.11.2058. View

11.

Sawchenko P, Friedman M . Sensory functions of the liver--a review. Am J Physiol. 1979; 236(1):R5-20. DOI: 10.1152/ajpregu.1979.236.1.R5. View

12.

Gretz N, Meisinger E, Strauch M . Partial nephrectomy and chronic renal failure: the 'mature' rat model. Contrib Nephrol. 1988; 60:46-55. DOI: 10.1159/000414789. View

13.

Kaye M . The effects in the rat of varying intakes of dietary calcium, phosphorus, and hydrogen ion on hyperparathyroidism due to chronic renal failure. J Clin Invest. 1974; 53(1):256-69. PMC: 301461. DOI: 10.1172/JCI107546. View

14.

Langer K, Frohling P, Diederich J, Brandl M, Lindenau K, Fekl W . Plasma amino and keto acids in chronic renal failure. Contrib Nephrol. 1988; 65:55-9. DOI: 10.1159/000415748. View

15.

Mitch W . Uremia and the control of protein metabolism. Nephron. 1988; 49(2):89-93. DOI: 10.1159/000185031. View

16.

Mitch W, Clark A . Specificity of the effects of leucine and its metabolites on protein degradation in skeletal muscle. Biochem J. 1984; 222(3):579-86. PMC: 1144218. DOI: 10.1042/bj2220579. View

17.

Pennisi A, Wang M, Kopple J . Effects of protein and amino acid diets in chronically uremic and control rats. Kidney Int. 1978; 13(6):472-9. DOI: 10.1038/ki.1978.70. View

18.

Lasserre J, Strauch M, Gretz N . Corticosterone: a catabolic factor in uremic rats with chronic acidosis?. Contrib Nephrol. 1990; 81:154-60. DOI: 10.1159/000418748. View

19.

Gretz N, Lasserre J, Langer K, Strauch M . A low-protein diet protects uremic rats against the negative sequelae of metabolic acidosis. Nephron. 1990; 54(4):327-33. DOI: 10.1159/000185889. View

20.

HAINES D, Swan C, Green J, Woodley J . Experimental uraemia with associated plasma amino acid abnormalities but without retarded food intake and weight gain. Nephron. 1989; 53(3):233-7. DOI: 10.1159/000185750. View