Isα-endorphin an Uremic Toxin?α-Endorphin Isolated from Filtrate of Uremic Patients with Carbohydrate Intolerance

Overview

Journal Amino Acids

Specialty Biochemistry

Date 2013 Nov 6

PMID 24190555

Citations 1

Authors

T Abiko

H Sekino

Affiliations

Soon will be listed here.

Abstract

A 16-residue peptide was isolated from filtrate of uremic patients with carbohydrate intolerance by ultrafiltration with an Amicon Centriflo DM-5 membrane, followed by gel-filtration on Sephadex G-50 and Sephadex G-25, droplet countercurrent chromatography and high performance liquid chromatography. The hexadecapeptide thus obtained was identical as the entire amino acid sequence ofα-endorphin by amino acid analysis, application of the Edman degradation analysis and measurement of physical constants and analytical data of the synthetic hexadecapeptide. This result seems to suggest that an accumulation ofα-endorphin in uremic patients might cause carbohydrate intolerance.

Citing Articles

Melanotropin-potentiating factor isolated from filtrate of uremic patients suffering from melanosis and carbohydrate intolerance.

Abiko T, Sekino H Amino Acids. 2013; 9(3):229-34.

PMID: 24178839 DOI: 10.1007/BF00805954.

References

WESTERVELT Jr F, SCHREINER G . The carbohydrate intolerance of uremic patients. Ann Intern Med. 1962; 57:266-76. DOI: 10.7326/0003-4819-57-2-266. View

Abiko T, Kumikawa M, Sekino H . Inhibition effect of rosette formation between human lymphocytes and sheep erythrocytes by specific heptapeptide isolated from uremic fluid and its analogs. Biochem Biophys Res Commun. 1979; 86(4):945-52. DOI: 10.1016/0006-291x(79)90209-2. View

Gajdos M, Dzurik R . Erythrocyte glycolysis in uraemia: cynamic balance caused by the opposite action of various factors. Int Urol Nephrol. 1974; 5(3):331-6. DOI: 10.1007/BF02081911. View

Grzeszczak W, KOKOT F, Dulawa J . Effects of naloxone administration on endocrine abnormalities in chronic renal failure. Am J Nephrol. 1987; 7(2):93-100. DOI: 10.1159/000167442. View

Furst P, Bergstrom J, Gordon A, Johnsson E, Zimmerman L . Separation of peptides of "middle" molecular weight from biological fluids of patients with uremia. Kidney Int Suppl. 1975; (3):272-5. View

BABB A, Farrell P, Uvelli D, Scribner B . Hemodialyzer evaluation by examination of solute molecular spectra. Trans Am Soc Artif Intern Organs. 1972; 18(0):98-105, 122. DOI: 10.1097/00002480-197201000-00030. View

WALEY S, Watson J . The action of trypsin on polylysine. Biochem J. 1953; 55(2):328-37. PMC: 1269242. DOI: 10.1042/bj0550328. View

Abiko T, Kumikawa M, Higuchi H, Sekino H . Identification and synthesis of a heptapeptide in uremic fluid. Biochem Biophys Res Commun. 1978; 84(1):184-94. DOI: 10.1016/0006-291x(78)90280-2. View

Asaba H, Furst P, Oules R, Yahiel V, Zimmerman L, Bergstrom J . The effect of hemodialysis on endogenous middle molecules in uremic patients. Clin Nephrol. 1979; 11(5):257-66. View

10.

PARTRIDGE S . Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948; 42(2):238-50. PMC: 1258661. DOI: 10.1042/bj0420238. View

11.

Kaiser E, Colescott R, Bossinger C, Cook P . Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. Anal Biochem. 1970; 34(2):595-8. DOI: 10.1016/0003-2697(70)90146-6. View

12.

Nakao K, Nakai Y, Oki S, Matsubara S, Konishi T, Nishitani H . Immunoreactive beta-endorphin in human cerebrospinal fluid. J Clin Endocrinol Metab. 1980; 50(2):230-3. DOI: 10.1210/jcem-50-2-230. View

13.

Abiko T, Kumikawa M, Ishizaki M, Takahashi H, Sekino H . Identification and synthesis of a tripeptide in ECUM fluid of an uremic patient. Biochem Biophys Res Commun. 1978; 83(2):357-64. DOI: 10.1016/0006-291x(78)90998-1. View

14.

Abiko T, ONODERA I, Sekino H . Characterization of an acidic tripeptide in neurotoxic dialysate. Chem Pharm Bull (Tokyo). 1980; 28(5):1629-33. DOI: 10.1248/cpb.28.1629. View

15.

Abiko T, ONODERA I, Sekino H . Isolation, structure and biological activity of the Trp-containing pentapeptide from uremic fluid. Biochem Biophys Res Commun. 1979; 89(3):813-21. DOI: 10.1016/0006-291x(79)91851-5. View