Investigating the Genetics of Bti Resistance Using MRNA Tag Sequencing: Application on Laboratory Strains and Natural Populations of the Dengue Vector Aedes Aegypti

Overview

Journal Evol Appl

Specialty Biology

Date 2013 Nov 5

PMID 24187584

Citations 6

Authors

Margot Paris

Sebastien Marcombe

Eric Coissac

Vincent Corbel

Jean-Philippe David

Laurence Despres

Affiliations

Soon will be listed here.

Abstract

Mosquito control is often the main method used to reduce mosquito-transmitted diseases. In order to investigate the genetic basis of resistance to the bio-insecticide Bacillus thuringiensis subsp. israelensis (Bti), we used information on polymorphism obtained from cDNA tag sequences from pooled larvae of laboratory Bti-resistant and susceptible Aedes aegypti mosquito strains to identify and analyse 1520 single nucleotide polymorphisms (SNPs). Of the 372 SNPs tested, 99.2% were validated using DNA Illumina GoldenGate® array, with a strong correlation between the allelic frequencies inferred from the pooled and individual data (r = 0.85). A total of 11 genomic regions and five candidate genes were detected using a genome scan approach. One of these candidate genes showed significant departures from neutrality in the resistant strain at sequence level. Six natural populations from Martinique Island were sequenced for the 372 tested SNPs with a high transferability (87%), and association mapping analyses detected 14 loci associated with Bti resistance, including one located in a putative receptor for Cry11 toxins. Three of these loci were also significantly differentiated between the laboratory strains, suggesting that most of the genes associated with resistance might differ between the two environments. It also suggests that common selected regions might harbour key genes for Bti resistance.

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References

Fernandez L, Aimanova K, Gill S, Bravo A, Soberon M . A GPI-anchored alkaline phosphatase is a functional midgut receptor of Cry11Aa toxin in Aedes aegypti larvae. Biochem J. 2005; 394(Pt 1):77-84. PMC: 1386005. DOI: 10.1042/BJ20051517. View

Georghiou G, Wirth M . Influence of Exposure to Single versus Multiple Toxins of Bacillus thuringiensis subsp. israelensis on Development of Resistance in the Mosquito Culex quinquefasciatus (Diptera: Culicidae). Appl Environ Microbiol. 1997; 63(3):1095-101. PMC: 1389136. DOI: 10.1128/aem.63.3.1095-1101.1997. View

Paris M, David J, Despres L . Fitness costs of resistance to Bti toxins in the dengue vector Aedes aegypti. Ecotoxicology. 2011; 20(6):1184-94. DOI: 10.1007/s10646-011-0663-8. View

Wang S, Sha Z, Sonstegard T, Liu H, Xu P, Somridhivej B . Quality assessment parameters for EST-derived SNPs from catfish. BMC Genomics. 2008; 9:450. PMC: 2570692. DOI: 10.1186/1471-2164-9-450. View

Paris M, Melodelima C, Coissac E, Tetreau G, Reynaud S, David J . Transcription profiling of resistance to Bti toxins in the mosquito Aedes aegypti using next-generation sequencing. J Invertebr Pathol. 2011; 109(2):201-8. DOI: 10.1016/j.jip.2011.11.004. View

Verlaan D, Ge B, Grundberg E, Hoberman R, C L Lam K, Koka V . Targeted screening of cis-regulatory variation in human haplotypes. Genome Res. 2008; 19(1):118-27. PMC: 2612965. DOI: 10.1101/gr.084798.108. View

Le Corre V, Kremer A . The genetic differentiation at quantitative trait loci under local adaptation. Mol Ecol. 2012; 21(7):1548-66. DOI: 10.1111/j.1365-294X.2012.05479.x. View

Kofler R, Pandey R, Schlotterer C . PoPoolation2: identifying differentiation between populations using sequencing of pooled DNA samples (Pool-Seq). Bioinformatics. 2011; 27(24):3435-6. PMC: 3232374. DOI: 10.1093/bioinformatics/btr589. View

Bonin A . Population genomics: a new generation of genome scans to bridge the gap with functional genomics. Mol Ecol. 2008; 17(16):3583-4. DOI: 10.1111/j.1365-294X.2008.03854.x. View

10.

Likitvivatanavong S, Chen J, Bravo A, Soberon M, Gill S . Cadherin, alkaline phosphatase, and aminopeptidase N as receptors of Cry11Ba toxin from Bacillus thuringiensis subsp. jegathesan in Aedes aegypti. Appl Environ Microbiol. 2010; 77(1):24-31. PMC: 3019721. DOI: 10.1128/AEM.01852-10. View

11.

Bravo A, Gill S, Soberon M . Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 2007; 49(4):423-35. PMC: 1857359. DOI: 10.1016/j.toxicon.2006.11.022. View

12.

Milano I, Babbucci M, Panitz F, Ogden R, Nielsen R, Taylor M . Novel tools for conservation genomics: comparing two high-throughput approaches for SNP discovery in the transcriptome of the European hake. PLoS One. 2011; 6(11):e28008. PMC: 3222667. DOI: 10.1371/journal.pone.0028008. View

13.

Gahan L, Gould F, Heckel D . Identification of a gene associated with Bt resistance in Heliothis virescens. Science. 2001; 293(5531):857-60. DOI: 10.1126/science.1060949. View

14.

Pigott C, Ellar D . Role of receptors in Bacillus thuringiensis crystal toxin activity. Microbiol Mol Biol Rev. 2007; 71(2):255-81. PMC: 1899880. DOI: 10.1128/MMBR.00034-06. View

15.

Taylor A . Aminopeptidases: structure and function. FASEB J. 1993; 7(2):290-8. DOI: 10.1096/fasebj.7.2.8440407. View

16.

Albert T, Molla M, Muzny D, Nazareth L, Wheeler D, Song X . Direct selection of human genomic loci by microarray hybridization. Nat Methods. 2007; 4(11):903-5. DOI: 10.1038/nmeth1111. View

17.

Librado P, Rozas J . DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009; 25(11):1451-2. DOI: 10.1093/bioinformatics/btp187. View

18.

Zhang R, Hua G, Andacht T, Adang M . A 106-kDa aminopeptidase is a putative receptor for Bacillus thuringiensis Cry11Ba toxin in the mosquito Anopheles gambiae. Biochemistry. 2008; 47(43):11263-72. PMC: 2660596. DOI: 10.1021/bi801181g. View

19.

Canovas A, Rincon G, Islas-Trejo A, Wickramasinghe S, Medrano J . SNP discovery in the bovine milk transcriptome using RNA-Seq technology. Mamm Genome. 2010; 21(11-12):592-8. PMC: 3002166. DOI: 10.1007/s00335-010-9297-z. View

20.

Jurat-Fuentes J, Adang M . Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae. Eur J Biochem. 2004; 271(15):3127-35. DOI: 10.1111/j.1432-1033.2004.04238.x. View