Ethanol Stimulates Phospholipid Turnover and Inositol 1,4,5-trisphosphate Production in Chlamydomonas Eugametos Gametes

Overview

Journal Planta

Specialty Biology

Date 2013 Nov 5

PMID 24186742

Citations 3

Authors

A Musgrave

H Kuin

M Jongen

P de Wildt

F Schuring

H Klerk

H van den Ende

Affiliations

Soon will be listed here.

Abstract

Alcohols induce mating-structure activation in Chlamydomonas eugametos gametes. From the effect of ethanol on the (32)P-labelling of polyphosphoinositides, we conclude that the synthesis of these lipids is stimulated. Biologically inactive concentrations of ethanol (<6%) had no effect on synthesis, but 6-8% ethanol stimulated synthesis for upto 60 min. The (32)P incorporated into polyphosphoinositides and phosphatidic acid during ethanol treatment was readily chased out when 1 mM unlabelled Na3PO4 was added. Using a binding assay for inositol 1,4,5-trisphosphate, we show that the production of this phospholipid constituent is dramatically increased after ethanol treatment. This effect, coupled to a rise in intracellular calcium concentration, could explain gamete activation. The significance of these results in explaining other ethanol-induced phenomena in algae is discussed.

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