Allele-specific Suppression of a Cell Cycle Mutant of S. Cerevisiae

A cell cycle (cdc) mutant of Saccharomyces cerevisiae is described which fails to complement cdc27-1 described by Hartwell et al. 1973), and is designated cdc27-47. Whereas cdc27-1 behaves as a single Mendelian gene (Hartwell et al. 1973), cdc27-47 requires the presence of an additional unlinked gene for expression of temperature-sensitivity. This gene, designated sts47, is present in some, but not all, laboratory wild-type strains. Expression of cdc27-1 is not influenced by STS47. A model for suppression is proposed involving the modification of conformation within a structural or enzyme complex.