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Killer DNA Plasmids of the Yeast Kluyveromyces Lactis : III. Plasmid Recombination

Overview
Journal Curr Genet
Specialty Genetics
Date 2013 Nov 5
PMID 24186296
Citations 4
Authors
M Wesolowski
A Algeri
H Fukuhara
Affiliations
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Abstract

The killer character of Kluyveromyces lactis is cytoplasmically inherited. Two DNA plasmids k1 and k2 (8.8 and 13.4 kilobase pairs, respectively) are associated with the killer system. Either cytoplasmic or nuclear mutations can lead to a loss of the killer activity. Two cytoplasmic non-killer mutants have been specifically studied. When they were crossed to each other, the killer phenotype was restored. Genetic analysis led us to conclude that each of the two mutants carried a mutation in the k1 plasmid and that the recovery of killer phenotype was due to genetic recombination of the plasmids, and not to complementation.

Citing Articles

Killer DNA Plasmids of the Yeast Kluyveromyces lactis : II. Restriction Endonuclease Maps.

Wesolowski M, Dumazert P, Fukuhara H Curr Genet. 2013; 5(3):199-203.

PMID: 24186295 DOI: 10.1007/BF00391806.

Killer DNA Plasmids of the Yeast Kluyveromyces lactis : I. Mutations Affecting the Killer Phenotype.

Wesolowski M, Algeri A, Goffrini P, Fukuhara H Curr Genet. 2013; 5(3):191-7.

PMID: 24186294 DOI: 10.1007/BF00391805.

Terminal segment of Kluyveromyces lactis linear DNA plasmid pGKL2 supports autonomous replication of hybrid plasmids in Saccharomyces cerevisiae.

Fujimura H, Hishinuma F, Gunge N Curr Genet. 1987; 12(2):99-104.

PMID: 3449223 DOI: 10.1007/BF00434663.

Heterologous gene expression on the linear DNA killer plasmid from Kluyveromyces lactis.

Kamper J, Esser K, Gunge N, Meinhardt F Curr Genet. 1991; 19(2):109-18.

PMID: 2065362 DOI: 10.1007/BF00326291.

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Wesolowski M, Algeri A, Goffrini P, Fukuhara H . Killer DNA Plasmids of the Yeast Kluyveromyces lactis : I. Mutations Affecting the Killer Phenotype. Curr Genet. 2013; 5(3):191-7. DOI: 10.1007/BF00391805. View
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Wesolowski M, Dumazert P, Fukuhara H . Killer DNA Plasmids of the Yeast Kluyveromyces lactis : II. Restriction Endonuclease Maps. Curr Genet. 2013; 5(3):199-203. DOI: 10.1007/BF00391806. View