Interactions Among Genes Controlling Sensitivity to Radiation (RAD) and to Alkylation by Nitrogen Mustard (SNM) in Yeast

Overview

Journal Curr Genet

Specialty Genetics

Date 2013 Nov 5

PMID 24186085

Citations 16

Authors

W Siede

M Brendel

Affiliations

Soon will be listed here.

Abstract

Three haploid yeast mutants (snm) sensitive or thermoconditionally sensitive to the DNA cross-linking agent nitrogen mustard (HN2) were crossed with four rad strains representing mutations in the three pathways of DNA dark repair. The resulting haploid double and triple mutant strains were tested for their sensitivity to UV, HN2 and HN1. From the observed epistatic or synergistic interactions of the combinations of mutant alleles we could derive the relation of the SNM1 and SNM2 genes to the postulated repair pathways. Alleles snm1-1 and snml-2 (ts) were found epistatic to genes of the rad3 group, whereas snm2-1 (ts) was epistatic to rad6. The snm1 and snm2 mutant alleles interacted synergistically. From these data it is concluded that the SNM1 gene product plays a cross-link specific role in excision repair while the SNM2 gene product may be involved in a system of error-prone repair.

Citing Articles

Mutant Gene snm2-1 (ts), conferring thermoconditional mutagen sensitivity in Saccharomyces cerevisiae, is allelic with RAD5.

Siedel W, Brendel M Curr Genet. 2013; 5(2):93-5.

PMID: 24186224 DOI: 10.1007/BF00365699.

Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.

Wang A, Sengerova B, Cattell E, Inagawa T, Hartley J, Kiakos K Genes Dev. 2011; 25(17):1859-70.

PMID: 21896658 PMC: 3175721. DOI: 10.1101/gad.15699211.

Disruption of mouse SNM1 causes increased sensitivity to the DNA interstrand cross-linking agent mitomycin C.

Dronkert M, de Wit J, Boeve M, Vasconcelos M, van Steeg H, Tan T Mol Cell Biol. 2000; 20(13):4553-61.

PMID: 10848582 PMC: 85844. DOI: 10.1128/MCB.20.13.4553-4561.2000.

Repair of intermediate structures produced at DNA interstrand cross-links in Saccharomyces cerevisiae.

McHugh P, Sones W, Hartley J Mol Cell Biol. 2000; 20(10):3425-33.

PMID: 10779332 PMC: 85635. DOI: 10.1128/MCB.20.10.3425-3433.2000.

Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links.

Wolter R, Siede W, Brendel M Mol Gen Genet. 1996; 250(2):162-8.

PMID: 8628215 DOI: 10.1007/BF02174175.

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