Organization and Expression of a TRNA Gene Cluster in Saccharomyces Cerevisiae Mitochondrial DNA

Overview

Journal Curr Genet

Specialty Genetics

Date 2013 Nov 5

PMID 24185959

Citations 1

Authors

D L Miller

N C Martin

Affiliations

Soon will be listed here.

Abstract

We have studied the organization and expression of a group of tRNA genes located on a 2,700 base pair portion of the yeast mitochondrial genome between the genetic markers cap (chloramphenicol resistance) and oxil (cytochrome oxidase subunit II). This region is spanned by mitochondrial DNA inserts of two recombinant plasmids, pYm162 and pYm267, which have been extensively mapped and sequenced. This tRNA group is composed of six tRNA genes, coding for tRNA AAR (Lys) , tRNA AGR (Arg) , tRNA GGN (Gly) , tRNA GAY (Asp) , tRNA AGY (Ser) , and tRNA CGN (Arg) . We report the sequence for the majority of the 2,700 base pair region including the genes for all six tRNAs. All six genes are oriented in the same direction and are, therefore, transcribed from the same DNA strand. Further, a comparison of the organization of this region with the analogous region of a related wild type strain shows that the tRNA gene order in the two strains is the same. Five of the six tRNA genes have corresponding transcripts in wild type RNA. Although a potential structural gene for tRNA CGN (Arg) is present, we do not detect a tRNA CGN (Arg) gene transcript.

Citing Articles

Characterization of a yeast mitochondrial locus necessary for tRNA biosynthesis. Deletion mapping and restriction mapping studies.

Miller D, Ross N, Fukuhara H, Martin N Mol Gen Genet. 1983; 191(3):512-8.

PMID: 6355772 DOI: 10.1007/BF00425771.

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