Specific Discrimination of Three Pathogenic Salmonella Enterica Subsp. Enterica Serotypes by CarB-based Oligonucleotide Microarray

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2013 Nov 5

PMID 24185846

Citations 1

Authors

Hwa Hui Shin

Byeong Hee Hwang

Jeong Hyun Seo

Hyung Joon Cha

Affiliations

Soon will be listed here.

Abstract

It is important to rapidly and selectively detect and analyze pathogenic Salmonella enterica subsp. enterica in contaminated food to reduce the morbidity and mortality of Salmonella infection and to guarantee food safety. In the present work, we developed an oligonucleotide microarray containing duplicate specific capture probes based on the carB gene, which encodes the carbamoyl phosphate synthetase large subunit, as a competent biomarker evaluated by genetic analysis to selectively and efficiently detect and discriminate three S. enterica subsp. enterica serotypes: Choleraesuis, Enteritidis, and Typhimurium. Using the developed microarray system, three serotype targets were successfully analyzed in a range as low as 1.6 to 3.1 nM and were specifically discriminated from each other without nonspecific signals. In addition, the constructed microarray did not have cross-reactivity with other common pathogenic bacteria and even enabled the clear discrimination of the target Salmonella serotype from a bacterial mixture. Therefore, these results demonstrated that our novel carB-based oligonucleotide microarray can be used as an effective and specific detection system for S. enterica subsp. enterica serotypes.

Citing Articles

Multiplex 16S rRNA-derived geno-biochip for detection of 16 bacterial pathogens from contaminated foods.

Shin H, Hwang B, Cha H Biotechnol J. 2016; 11(11):1405-1414.

PMID: 27492058 PMC: 5132059. DOI: 10.1002/biot.201600043.

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