Comparative RFLP Mapping of Hordeum Vulgare and Triticum Tauschii

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 2

PMID 24178097

Citations 9

Authors

D M Namuth

N L Lapitan

K S Gill

B S Gill

Affiliations

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Abstract

Hordeum vulgare (barley) and Triticum tauschii are related, but sexually incompatible, species. This study was conducted to determine the extent of homology between the genomes of barley and T. tauschii using a common set of restriction fragment length polymorphism (RFLP) markers. Results showed that >95% of low-copy sequences are shared, but 42% of the conserved sequences showed copy-number differences. Sixty-three loci were mapped in T. tauschii using RFLP markers previously mapped in barley. A comparison of RFLP marker order showed that, in general, barley and T. tauschii have conserved linkage groups, with markers in the same linear orders. However, six of the seven linkage groups of T. tauschii contained markers which mapped to unrelated (i.e., non-homoeologous) barley chromosomes. Additionally, four of the T. tauschii linkage groups contained markers that were switched in order with respect to barley. All the chromosome segments differing between T. tauschii and barley contained markers that were detected by multi-copy probes. The results suggest that the observed differences between the T. tauschii and barley genomes were brought about by duplications or deletions of segments in one or both species. The implications of these findings for genetic mapping, breeding, and plant genome evolution are discussed.

Citing Articles

A barley RFLP map: alignment of three barley maps and comparisons to Gramineae species.

Sherman J, Fenwick A, Namuth D, Lapitan N Theor Appl Genet. 2013; 91(4):681-90.

PMID: 24169898 DOI: 10.1007/BF00223297.

A molecular linkage map of rye.

Loarce Y, Hueros G, Ferrer E Theor Appl Genet. 2013; 93(7):1112-8.

PMID: 24162489 DOI: 10.1007/BF00230133.

RFLP mapping of three new loci for resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) in barley.

Schonfeld M, Ragni A, Fischbeck G, Jahoor A Theor Appl Genet. 2013; 93(1-2):48-56.

PMID: 24162198 DOI: 10.1007/BF00225726.

High-resolution mapping of the Alp locus and identification of a candidate gene HvMATE controlling aluminium tolerance in barley (Hordeum vulgare L.).

Wang J, Raman H, Zhou M, Ryan P, Delhaize E, Hebb D Theor Appl Genet. 2007; 115(2):265-76.

PMID: 17551710 DOI: 10.1007/s00122-007-0562-9.

Chromosomal rearrangements differentiating the ryegrass genome from the Triticeae, oat, and rice genomes using common heterologous RFLP probes.

Sim S, Chang T, Curley J, Warnke S, Barker R, Jung G Theor Appl Genet. 2005; 110(6):1011-9.

PMID: 15742203 DOI: 10.1007/s00122-004-1916-1.

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