Molecular Analysis of the RNA and Protein Components Recognized by Anti-La(SS-B) Autoantibodies

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1985 Dec 1

PMID 2417765

Citations 5

Authors

L J McNeilage

S Whittingham

I JACK

I R Mackay

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to determine whether sera with autoantibodies to the La(SS-B) nuclear antigen react with the same or different sets of cellular or viral ribonucleoproteins (RNPs) and whether patients with anti-La(SS-B) comprised a homogeneous group with respect to phenotypic and serological markers. The 34 anti-La(SS-B) sera studied were detected in the course of screening 2,000 sera referred from patients with suspected or defined multisystem autoimmune disease. Analysis of the molecular components of the small nuclear (sn) RNPs isolated from immune complexes developed in vitro between the IgG fractions of the anti-La(SS-B) sera and cell lines selected for their content of viral and cellular (non-viral) RNA showed that all 34 anti-La(SS-B) sera reacted with the same group of cellular RNAs and with two viral RNAs encoded by Epstein-Barr virus. The La(SS-B) RNPs contained one major 50,000 dalton antigenic polypeptide that resolved into 5-6 heterogeneously charged isospecies on two-dimensional immunoblots. In addition to anti-La(SS-B) reactivity, all 34 sera were shown to contain anti-Ro(SS-A) activity by counterimmunoelectrophoresis (CIEP); however, with three exceptions, the antigenic Ro(SS-A) polypeptide was not detectable by immunoblotting. The homogeneity of this group with anti-La(SS-B) was indicated by the findings that of the 34 cases 31 (88%) had hypergammaglobulinaemia, 33 (97%) had rheumatoid factor and 27 (of 30 tested, 90%) were HLA-B8. Thus all anti-La(SS-B) sera react with the same set of RNAs associated with an antigenic 50,000 dalton nucleoprotein, and the presence of anti-La(SS-B) autoantibodies identified a homogeneous group of patients with the serological and phenotypic features of primary Sjögren's syndrome.

Citing Articles

Ultrastructural immunogold cytochemistry with autoimmune human sera and an antibody to uridine implicate human mast cell granules in RNA biology.

Dvorak A, Morgan E Histochem J. 2001; 32(11):685-96.

PMID: 11272809 DOI: 10.1023/a:1004119500801.

Human autoantibodies directed against the RNA recognition motif of La (SS-B) bind to a conformational epitope present on the intact La (SS-B)/Ro (SS-A) ribonucleoprotein particle.

Rischmueller M, McNeilage L, McCluskey J, Gordon T Clin Exp Immunol. 1995; 101(1):39-44.

PMID: 7542577 PMC: 1553316. DOI: 10.1111/j.1365-2249.1995.tb02274.x.

Heterogeneity of the Ro/SSA antigen. Different molecular forms in lymphocytes and red blood cells.

Rader M, OBrien C, Liu Y, Harley J, Reichlin M J Clin Invest. 1989; 83(4):1293-8.

PMID: 2784800 PMC: 303820. DOI: 10.1172/JCI114014.

Association of La and Ro antigens with intracellular structures in HEp-2 carcinoma cells.

Bachmann M, Mayet W, Schroder H, Pfeifer K, Meyer zum Buschenfelde K, Muller W Proc Natl Acad Sci U S A. 1986; 83(20):7770-4.

PMID: 2429318 PMC: 386803. DOI: 10.1073/pnas.83.20.7770.

Definition of a discontinuous immunodominant epitope at the NH2 terminus of the La/SS-B ribonucleoprotein autoantigen.

McNeilage L, Umapathysivam K, Macmillan E, Guidolin A, Whittingham S, Gordon T J Clin Invest. 1992; 89(5):1652-6.

PMID: 1373741 PMC: 443042. DOI: 10.1172/JCI115762.

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