Alpha 2.0
Login Register
» Articles » PMID: 24177534

Homoplasmic Yeast Cells Contain No Selectable "hidden" Mitochondrial Alleles

Overview
Journal Curr Genet
Specialty Genetics
Date 2013 Nov 2
PMID 24177534
Citations 2
Authors
J E Lewis
C W Birky Jr
Affiliations
Soon will be listed here.
Abstract

Zygotes of Saccharomyces cerevisiae that are heteroplasmic for mitochondrial alleles produce diploid progeny that are homoplasmic for one allele or the other, judged by the criterion that upon further subcloning they produce daughter cells of only one phenotype or the other. Here we show that when such cells are subjected to strong selection for the missing allele, it cannot be detected, so that it is probably not present in even a single copy.

Citing Articles

Chloroplast gene suppression of defective ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardii: evidence for stable heteroplasmic genes.

Spreitzer R, Chastain C, Ogren W Curr Genet. 2013; 9(1):83-9.

PMID: 24173514 DOI: 10.1007/BF00396208.

The origin of mutant cells: mechanisms by which Saccharomyces cerevisiae produces cells homoplasmic for new mitochondrial mutations.

Backer J, Birky Jr C Curr Genet. 1985; 9(8):627-40.

PMID: 3916732 DOI: 10.1007/BF00449815.

References
1.
Birky Jr C . Relaxed cellular controls and organelle heredity. Science. 1983; 222(4623):468-75. DOI: 10.1126/science.6353578. View
2.
Backer J, Birky Jr C . The origin of mutant cells: mechanisms by which Saccharomyces cerevisiae produces cells homoplasmic for new mitochondrial mutations. Curr Genet. 1985; 9(8):627-40. DOI: 10.1007/BF00449815. View
3.
Thrailkill K, Birky Jr C . Intracellular population genetics: evidence for random drift of mitochondrial allele frequencies in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Genetics. 1980; 96(1):237-62. PMC: 1214292. DOI: 10.1093/genetics/96.1.237. View
4.
Knight J . New antibiotic resistance Loci in the ribosomal region of yeast mitochondrial DNA. Genetics. 1980; 94(1):69-92. PMC: 1214138. DOI: 10.1093/genetics/94.1.69. View
5.
Birky Jr C . On the origin of mitochondrial mutants: evidence for intracellular selection of mitochondria in the origin of antibiotic-resistant cells in yeast. Genetics. 1973; 74(3):421-32. PMC: 1212959. DOI: 10.1093/genetics/74.3.421. View