Monoclonal Antibodies Defining Blood Group A Variants with Difucosyl Type 1 Chain (ALeb) and Difucosyl Type 2 Chain (ALey)

Overview

Journal Biochemistry

Specialty Biochemistry

Date 1985 Oct 22

PMID 2417620

Citations 4

Authors

H Clausen

J M McKIBBIN

S Hakomori

Affiliations

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Abstract

Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the difucosyl type 2 chain A (ALey) and does not react with the difucosyl type 1 chain or monofucosyl type 2 chain. Antibody HH3 (IgG2a) reacts exclusively with the difucosyl type 1 chain A (ALeb) and does not react with the monofucosyl type 1 chain A or mono- and difucosyl type 2 chain A. These hybridoma antibodies were obtained by immunization of mice with purified glycolipid antigens and were selected by their reactivity with the specific glycolipid structures. These antibodies, together with previously established monoclonal antibody AH-21, specific for monofucosyl type 1 chain A, and monoclonal antibody TH-1, specific for type 3 chain A, are extremely useful to define blood group A variants present in cells and tissues.

Citing Articles

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Tanaka K, Suzuki A, Aoki D, Iwamori M Glycoconj J. 2019; 36(3):219-226.

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Blood group glycosphingolipid expression in kidney of an individual with the rare blood group A1 Le(a-b+) p phenotype: absence of blood group structures based on the globoseries.

Lindstrom K, Jovall P, Ghardashkani S, Samuelsson B, Breimer M Glycoconj J. 1996; 13(2):307-13.

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Regulation of the oncodevelopmental expression of type 1 chain ABH and Lewis(b) blood group antigens in human colon by alpha-2-L-fucosylation.

Orntoft T, Greenwell P, Clausen H, WATKINS W Gut. 1991; 32(3):287-93.

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