Membrane Structure: Morphological and Chemical Alterations in Phospholipase-C-treated Mitochondria and Red Cell Ghosts

Overview

Journal J Membr Biol

Date 2013 Nov 1

PMID 24174146

Citations 4

Authors

A C Ottolenghi

M H Bowman

Affiliations

Soon will be listed here.

Abstract

Phospholipase C fromBacillus cereus was used as a tool for the study of membrane structure. Interference and electron microscopic examination of isolated rat kidney mitochondria and human red blood cell ghosts incubated in the presence of the enzyme revealed that there were discrete areas of digestion on the membranes. These areas were distributed over the surface of the membrane but did not coalesce. These data are interpreted as giving support to the membrane model which postulates that protein does not cover the entire phospholipid layer of the membrane, but that there are areas of the membrane in which the phospholipid polar groups are in direct contact with the outside milieu.

Citing Articles

Effects of phospholipase C on human erythrocytes.

Bowman M, Ottolenghi A, Mengel C J Membr Biol. 2013; 4(1):156-64.

PMID: 24174236 DOI: 10.1007/BF02431968.

Ultrastructural observations on the cell surface of the intestinal epithelium of the nematode, Ascaris suum. Nature of the electronegative charge.

Trimble 3rd J, Thompson S Cell Tissue Res. 1980; 205(1):55-65.

PMID: 6153929 DOI: 10.1007/BF00234442.

Protein and protein-lipid membranes from solubilized erythrocyte ghosts.

Pohl G Biophysik. 1971; 7(3):236-44.

PMID: 5092558 DOI: 10.1007/BF01190731.

Effect of Clostridium perfringens phospholipase C(alpha-toxin) on the human diploid fibroblast membrane.

Mollby R, Thelestam M, Wadstrom T J Membr Biol. 1974; 16(4):313-30.

PMID: 4366075 DOI: 10.1007/BF01872421.

References

Korn E . Structure of biological membranes. Science. 1966; 153(3743):1491-8. DOI: 10.1126/science.153.3743.1491. View

Macfarlane M . The biochemistry of bacterial toxins: 2. The enzymic specificity of Clostridium welchii lecithinase. Biochem J. 1948; 42(4):587-90. PMC: 1258785. View

Ottolenghi A . Cationic antibiotics and phospholipase C as tools in the study of phospholipid structure and function. II. Combined effects of colistin sulfate and phospholipase C on the in vitro blood clotting system. Can J Biochem. 1967; 45(2):245-9. DOI: 10.1139/o67-027. View

Finean J, Martonosi A . The action of phospholipase C on muscle microsomes: a correlation of electron microscope and biochemical data. Biochim Biophys Acta. 1965; 98(3):547-53. DOI: 10.1016/0005-2760(65)90151-7. View

Lenard J, Singer S . Structure of membranes: reaction of red blood cell membranes with phospholipase C. Science. 1968; 159(3816):738-9. DOI: 10.1126/science.159.3816.738-a. View

Wallach D, Zahler P . Protein conformations in cellular membranes. Proc Natl Acad Sci U S A. 1966; 56(5):1552-9. PMC: 220029. DOI: 10.1073/pnas.56.5.1552. View

LOWRY O, ROBERTS N, LEINER K, Wu M, FARR A . The quantitative histochemistry of brain. I. Chemical methods. J Biol Chem. 1954; 207(1):1-17. View

LOCKWOOD W . A RELIABLE AND EASILY SECTIONED EPOXY EMBEDDING MEDIUM. Anat Rec. 1964; 150:129-39. DOI: 10.1002/ar.1091500204. View

DODGE J, Mitchell C, HANAHAN D . The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963; 100:119-30. DOI: 10.1016/0003-9861(63)90042-0. View

10.

Green D, Perdue J . Membranes as expressions of repeating units. Proc Natl Acad Sci U S A. 1966; 55(5):1295-302. PMC: 224315. DOI: 10.1073/pnas.55.5.1295. View

11.

Scarpelli D . EXPERIMENTAL NEPHROCALCINOSIS. A BIOCHEMICAL AND MORPHOLOGIC STUDY. Lab Invest. 1965; 14:123-41. View

12.

Grossman I, Heitkamp D, SACKTOR B . Morphologic and biochemical effects of Clostridium perfringens alpha toxin on intact and isolated skeletal muscle mitochondria. Am J Pathol. 1967; 50(1):77-88. PMC: 1965171. View

13.

STRUNK S, Smith C, Blumberg J . Ultrastructural studies on the lesion produced in skeletal muscle fibers by crude type A Clostridium perfringens toxin and its purified alpha fraction. Am J Pathol. 1967; 50(1):89-107. PMC: 1965172. View

14.

Lenard J, Singer S . Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism. Proc Natl Acad Sci U S A. 1966; 56(6):1828-35. PMC: 220191. DOI: 10.1073/pnas.56.6.1828. View