Application of Two Microsatellite Sequences in Wheat Storage Proteins As Molecular Markers

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 1

PMID 24173898

Citations 29

Authors

K M Devos

G J Bryan

A J Collins

P STEPHENSON

M D Gale

Affiliations

Soon will be listed here.

Abstract

In eukaryotes, tandem arrays of simple-sequence repeat sequences can find applications as highly variable and multi-allelic PCR-based genetic markers. In hexaploid bread wheat, a large-genome inbreeding species with low levels of RFLP, di- and trinucleotide tandem repeats were found in 22 published gene sequences, two of which were converted to PCR-based markers. These were shown to be genome-specific and displayed high levels of variation. These characteristics make them especially suitable for intervarietal breeding applications.

Citing Articles

Genome-wide microsatellite characterization and their marker development and transferability in Broussonetia Species.

Jia X, Li H, Han Y, Wang L, Lai C, Liu X BMC Genomics. 2025; 26(1):61.

PMID: 39844044 PMC: 11755984. DOI: 10.1186/s12864-025-11238-0.

The genome of the oomycete Peronosclerospora sorghi, a cosmopolitan pathogen of maize and sorghum, is inflated with dispersed pseudogenes.

Fletcher K, Martin F, Isakeit T, Cavanaugh K, Magill C, Michelmore R G3 (Bethesda). 2023; 13(3).

PMID: 36592124 PMC: 9997571. DOI: 10.1093/g3journal/jkac340.

Identification and evaluation of flos introduced to the Hailuogou area based on ITS sequences and active compounds.

He H, Zhang D, Gao J, Andersen T, Mou Z PeerJ. 2019; 7:e7636.

PMID: 31534858 PMC: 6730534. DOI: 10.7717/peerj.7636.

Effect of Allelic Variation at the Loci on Breadmaking Quality Parameters in Hexaploid Wheat ( L.).

Bonafede M, Tranquilli G, Pfluger L, Pena R, Dubcovsky J J Cereal Sci. 2016; 62:143-150.

PMID: 27818572 PMC: 5096839. DOI: 10.1016/j.jcs.2015.02.001.

A QTL with major effect on reducing leaf rust severity on the short arm of chromosome 1A of wheat detected across different genetic backgrounds and diverse environments.

Du Z, Che M, Li G, Chen J, Quan W, Guo Y Theor Appl Genet. 2015; 128(8):1579-94.

PMID: 25982130 DOI: 10.1007/s00122-015-2533-x.

References

Pitts E, Rafalski J, Hedgcoth C . Nucleotide sequence and encoded amino acid sequence of a genomic gene region for a low molecular weight glutenin. Nucleic Acids Res. 1988; 16(23):11376. PMC: 339026. DOI: 10.1093/nar/16.23.11376. View

Moore S, Sargeant L, King T, Mattick J, Georges M, Hetzel D . The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics. 1991; 10(3):654-60. DOI: 10.1016/0888-7543(91)90448-n. View

Akkaya M, Bhagwat A, Cregan P . Length polymorphisms of simple sequence repeat DNA in soybean. Genetics. 1992; 132(4):1131-9. PMC: 1205234. DOI: 10.1093/genetics/132.4.1131. View

Senior M, Heun M . Mapping maize microsatellites and polymerase chain reaction confirmation of the targeted repeats using a CT primer. Genome. 1993; 36(5):884-9. DOI: 10.1139/g93-116. View

Weissenbach J, Gyapay G, Dib C, Vignal A, Morissette J, Millasseau P . A second-generation linkage map of the human genome. Nature. 1992; 359(6398):794-801. DOI: 10.1038/359794a0. View

McFADDEN E, SEARS E . The origin of Triticum spelta and its free-threshing hexaploid relatives. J Hered. 2010; 37:81 107. DOI: 10.1093/oxfordjournals.jhered.a105590. View

Zhao X, Kochert G . Phylogenetic distribution and genetic mapping of a (GGC)n microsatellite from rice (Oryza sativa L.). Plant Mol Biol. 1993; 21(4):607-14. DOI: 10.1007/BF00014544. View

Rafalski J . Structure of wheat gamma-gliadin genes. Gene. 1986; 43(3):221-9. DOI: 10.1016/0378-1119(86)90210-6. View

DOvidio R, Tanzarella O, Porceddu E . Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction. Plant Mol Biol. 1990; 15(1):169-71. DOI: 10.1007/BF00017737. View

10.

Morgante M, Olivieri A . PCR-amplified microsatellites as markers in plant genetics. Plant J. 1993; 3(1):175-82. View

11.

Lagercrantz U, Ellegren H, Andersson L . The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates. Nucleic Acids Res. 1993; 21(5):1111-5. PMC: 309270. DOI: 10.1093/nar/21.5.1111. View

12.

Bell C, Ecker J . Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics. 1994; 19(1):137-44. DOI: 10.1006/geno.1994.1023. View

13.

Kwok S, Kellogg D, McKinney N, Spasic D, Goda L, Levenson C . Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res. 1990; 18(4):999-1005. PMC: 330356. DOI: 10.1093/nar/18.4.999. View

14.

Wu K, Tanksley S . Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol Gen Genet. 1993; 241(1-2):225-35. DOI: 10.1007/BF00280220. View

15.

Sabelli P, Shewry P . Characterization and organization of gene families at the Gli-1 loci of bread and durum wheats by restriction fragment analysis. Theor Appl Genet. 2013; 83(2):209-16. DOI: 10.1007/BF00226253. View