Serum Antibodies Against βH-crystallins in the American Cocker Spaniel

Overview

Journal Vet Ophthalmol

Specialties Ophthalmology
Veterinary Medicine

Date 2013 Nov 1

PMID 24171837

Authors

Nobuyuki Kanemaki

Chiho Fukiage

Yoichiro Ichikawa

Thomas R Shearer

Mitsuyoshi Azuma

Affiliations

Soon will be listed here.

Abstract

Objective: To detect antibodies for lens βH-crystallins in the serum from the American Cocker Spaniel (ACS) presenting with and without cataracts and with and without uveitis.

Animal Studied: Seventy-three American Cocker Spaniels and six normal Beagles.

Procedures: Sera were collected from 73 ACSs, including those with normal lenses and those with cataracts, or uveitis. Fractionated, normal Beagle lens βH-crystallins were separated by one- or two-dimensional electrophoresis. The separated lens βH-crystallins were used on immunoblots as sentinel substrates against which the ACS sera were tested for the presence of antibodies against βH-crystallins.

Results: Sera from approximately two-thirds of study animals contained antibodies to some βH-crystallin polypeptides, but reactivity varied among patients. Contrary to some hypotheses, serum antibodies to groups of βH-crystallins did not relate to the stages of cataract. However, detailed analysis by two-dimensional immunoblotting and mass spectrometry showed that three spots originating from βA1-crystallin were detected only in sera from cataract patients.

Conclusion: Serum antibodies to βA1-crystallin may be associated with the development of cataract.

References

Merck K, Cruysberg J, Bloemendal H, de Jong W . Characterization of anti-crystallin autoantibodies in patients with cataract. Mol Biol Rep. 1993; 17(2):93-9. DOI: 10.1007/BF00996216. View

Dirks R, van Genesen S, Kruse J, Jorissen L, Lubsen N . Extralenticular expression of the rodent betaB2-crystallin gene. Exp Eye Res. 1998; 66(2):267-9. DOI: 10.1006/exer.1997.0439. View

Takata T, Oxford J, Brandon T, Lampi K . Deamidation alters the structure and decreases the stability of human lens betaA3-crystallin. Biochemistry. 2007; 46(30):8861-71. PMC: 2597435. DOI: 10.1021/bi700487q. View

Adkins E, Hendrix D . Outcomes of dogs presented for cataract evaluation: a retrospective study. J Am Anim Hosp Assoc. 2005; 41(4):235-40. DOI: 10.5326/0410235. View

Gelatt K, MacKay E . Prevalence of primary breed-related cataracts in the dog in North America. Vet Ophthalmol. 2005; 8(2):101-11. DOI: 10.1111/j.1463-5224.2005.00352.x. View

Wilmarth P, Tanner S, Dasari S, Nagalla S, Riviere M, Bafna V . Age-related changes in human crystallins determined from comparative analysis of post-translational modifications in young and aged lens: does deamidation contribute to crystallin insolubility?. J Proteome Res. 2006; 5(10):2554-66. PMC: 2536618. DOI: 10.1021/pr050473a. View

Leasure J, Gelatt K, Mackay E . The relationship of cataract maturity to intraocular pressure in dogs. Vet Ophthalmol. 2002; 4(4):273-6. DOI: 10.1046/j.1463-5216.2001.00199.x. View

DANIEL W, Noonan N, Gelatt K . Isolation and characterization of the crystallins of the normal and cataractous canine lens. Curr Eye Res. 1984; 3(7):911-22. DOI: 10.3109/02713688409167208. View

Ranjan M, Nayak S, Kosuri T, Rao B . Immunochemical detection of glycated lens crystallins and their circulating autoantibodies in human serum during aging. Mol Vis. 2008; 14:2056-66. PMC: 2584771. View

10.

Feng J, Smith D, Smith J . Human lens beta-crystallin solubility. J Biol Chem. 2000; 275(16):11585-90. DOI: 10.1074/jbc.275.16.11585. View

11.

Wang L, Liu D, Liu P, Yu Y . Proteomics analysis of water insoluble-urea soluble crystallins from normal and dexamethasone exposed lens. Mol Vis. 2012; 17:3423-36. PMC: 3249430. View

12.

Duncan G, Jacob T . Calcium and the physiology of cataract. Ciba Found Symp. 1984; 106:132-52. DOI: 10.1002/9780470720875.ch8. View

13.

Park S, Yi N, Jeong M, Kim W, Kim S, Chae J . Clinical manifestations of cataracts in small breed dogs. Vet Ophthalmol. 2009; 12(4):205-10. DOI: 10.1111/j.1463-5224.2009.00697.x. View

14.

Chan M, Dolinska M, Sergeev Y, Wingfield P, Hejtmancik J . Association properties of betaB1- and betaA3-crystallins: ability to form heterotetramers. Biochemistry. 2008; 47(42):11062-9. PMC: 2752815. DOI: 10.1021/bi8012438. View

15.

Robertson L, Morton J, Yamaguchi M, Bickerstaffe R, Shearer T, Azuma M . Calpain may contribute to hereditary cataract formation in sheep. Invest Ophthalmol Vis Sci. 2005; 46(12):4634-40. DOI: 10.1167/iovs.04-1291. View

16.

Williams D . Oxidation, antioxidants and cataract formation: a literature review. Vet Ophthalmol. 2006; 9(5):292-8. DOI: 10.1111/j.1463-5224.2006.00498.x. View

17.

Barros P, Angelotti A, Nobre F, Morales A, Fantoni D, Barros S . Antioxidant profile of cataractous English Cocker Spaniels. Vet Ophthalmol. 2001; 2(2):83-86. DOI: 10.1046/j.1463-5224.1999.00056.x. View

18.

Xi J, Farjo R, Yoshida S, Kern T, Swaroop A, Andley U . A comprehensive analysis of the expression of crystallins in mouse retina. Mol Vis. 2003; 9:410-9. View

19.

Ibaraki N, Lin L, Dang L, Reddy V, Singh D, Sueno T . Anti-beta-crystallin antibodies (mouse) or sera from humans with age-related cataract are cytotoxic for lens epithelial cells in culture. Exp Eye Res. 1997; 64(2):229-38. DOI: 10.1006/exer.1996.0203. View

20.

David L, Shearer T . Beta-crystallins insolubilized by calpain II in vitro contain cleavage sites similar to beta-crystallins insolubilized during cataract. FEBS Lett. 1993; 324(3):265-70. DOI: 10.1016/0014-5793(93)80131-d. View