Damage-dependent Regulation of MUS81-EME1 by Fanconi Anemia Complementation Group A Protein

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2013 Oct 31

PMID 24170812

Citations 12

Authors

Anaid Benitez

Fenghua Yuan

Satoshi Nakajima

Leizhen Wei

Liangyue Qian

Richard Myers

Jennifer J Hu

Li Lan

Yanbin Zhang

Affiliations

Soon will be listed here.

Abstract

MUS81-EME1 is a DNA endonuclease involved in replication-coupled repair of DNA interstrand cross-links (ICLs). A prevalent hypothetical role of MUS81-EME1 in ICL repair is to unhook the damage by incising the leading strand at the 3' side of an ICL lesion. In this study, we report that purified MUS81-EME1 incises DNA at the 5' side of a psoralen ICL residing in fork structures. Intriguingly, ICL repair protein, Fanconi anemia complementation group A protein (FANCA), greatly enhances MUS81-EME1-mediated ICL incision. On the contrary, FANCA exhibits a two-phase incision regulation when DNA is undamaged or the damage affects only one DNA strand. Studies using truncated FANCA proteins indicate that both the N- and C-moieties of the protein are required for the incision regulation. Using laser-induced psoralen ICL formation in cells, we find that FANCA interacts with and recruits MUS81 to ICL lesions. This report clarifies the incision specificity of MUS81-EME1 on ICL damage and establishes that FANCA regulates the incision activity of MUS81-EME1 in a damage-dependent manner.

Citing Articles

Head and Neck Cancer Susceptibility and Metabolism in Fanconi Anemia.

Chihanga T, Vicente-Munoz S, Ruiz-Torres S, Pal B, Sertorio M, Andreassen P Cancers (Basel). 2022; 14(8).

PMID: 35454946 PMC: 9025423. DOI: 10.3390/cancers14082040.

Making Choices: DNA Replication Fork Recovery Mechanisms.

Kondratick C, Washington M, Spies M Semin Cell Dev Biol. 2021; 113:27-37.

PMID: 33967572 PMC: 8098667. DOI: 10.1016/j.semcdb.2020.10.001.

Fanconi anemia pathway as a prospective target for cancer intervention.

Liu W, Palovcak A, Li F, Zafar A, Yuan F, Zhang Y Cell Biosci. 2020; 10:39.

PMID: 32190289 PMC: 7075017. DOI: 10.1186/s13578-020-00401-7.

Delineating the role of FANCA in glucose-stimulated insulin secretion in β cells through its protein interactome.

Lagundzin D, Hu W, Law H, Krieger K, Qiao F, Clement E PLoS One. 2019; 14(8):e0220568.

PMID: 31461451 PMC: 6713327. DOI: 10.1371/journal.pone.0220568.

Holding All the Cards-How Fanconi Anemia Proteins Deal with Replication Stress and Preserve Genomic Stability.

Datta A, Brosh Jr R Genes (Basel). 2019; 10(2).

PMID: 30813363 PMC: 6409899. DOI: 10.3390/genes10020170.

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