Molecular Mapping of the Arabidopsis Locus RPP11 Which Conditions Isolate-specific Hypersensitive Resistance Against Downy Mildew in Ecotype RLD

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Oct 30

PMID 24166179

Citations 3

Authors

H J Joos

B Mauch-Mani

A J Slusarenko

Affiliations

Soon will be listed here.

Abstract

Isolate WELA of the plant pathogenic oomycete fungus Peronospora parasitica causes downy mildew in the Arabidopsis thaliana ecotypes Weiningen (Wei-0) and La-er, whereas ecotypes RLD and Col-0 are resistant. Genetic crosses between resistant RLD and susceptible Wei-0 showed that resistance was inherited in a simple Mendelian fashion as a monogenic dominant trait. The interactions between different isolates of P. parasitica and ecotypes of A. thaliana show race-specific variation and fit a gene-for-gene relationship. The RPP11 resistance gene was mapped by following the co-segregation of the resistance phenotype with RFLP markers in a mapping population of 254 F3 families derived from RLD x Wei-0 F2 individuals. Linkage analysis using version 1.9 of the MAPMAKER program placed the RPP11 resistance locus on chromosome III between marker m249 (two recombinants) and marker g2534 (six recombinants). Markers g2534 and g4117 are on YAC EG7H1. Marker g4117 and one end probe (N5) generated from YAC EG7H1 showed no recombinants. The YAC end probe N5, which was generated by plasmid rescue, was used to screen clones in the Eric Ward YAC library and a YAC was fished (EW19B12) which also hybridised with m249. Thus, a YAC contig has been established over the region where the resistance locus maps. Because the YACs were made with ecotype Columbia DNA it is necessary to isolate the equivalent region from RLD in order to clone the resistance locus. To this end a phage λ-DASH (™) genomic library was prepared from RLD and a contig covering the relevant region of the YACs is currently under construction.

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References

Grill E, Somerville C . Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mol Gen Genet. 1991; 226(3):484-90. DOI: 10.1007/BF00260662. View

Lander E, Green P . Construction of multilocus genetic linkage maps in humans. Proc Natl Acad Sci U S A. 1987; 84(8):2363-7. PMC: 304651. DOI: 10.1073/pnas.84.8.2363. View

Chang C, Bowman J, DeJohn A, Lander E, Meyerowitz E . Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1988; 85(18):6856-60. PMC: 282077. DOI: 10.1073/pnas.85.18.6856. View

Debener T, Lehnackers H, Arnold M, Dangl J . Identification and molecular mapping of a single Arabidopsis thaliana locus determining resistance to a phytopathogenic Pseudomonas syringae isolate. Plant J. 2011; 1(3):289-302. DOI: 10.1046/j.1365-313X.1991.t01-7-00999.x. View

Staskawicz B, Ausubel F, Baker B, Ellis J, Jones J . Molecular genetics of plant disease resistance. Science. 1995; 268(5211):661-7. DOI: 10.1126/science.7732374. View

Bent A, Kunkel B, Dahlbeck D, Brown K, Schmidt R, Giraudat J . RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science. 1994; 265(5180):1856-60. DOI: 10.1126/science.8091210. View

Hwang I, Kohchi T, Hauge B, Goodman H, Schmidt R, Cnops G . Identification and map position of YAC clones comprising one-third of the Arabidopsis genome. Plant J. 1991; 1(3):367-74. DOI: 10.1046/j.1365-313x.1991.t01-5-00999.x. View

Nam H, Giraudat J, Den Boer B, Moonan F, Loos W, Hauge B . Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana. Plant Cell. 1989; 1(7):699-705. PMC: 159806. DOI: 10.1105/tpc.1.7.699. View

Koch E, Slusarenko A . Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell. 1990; 2(5):437-45. PMC: 159900. DOI: 10.1105/tpc.2.5.437. View

10.

Guzman P, Ecker J . Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast. Nucleic Acids Res. 1988; 16(23):11091-105. PMC: 338998. DOI: 10.1093/nar/16.23.11091. View

11.

Jarvis P, Lister C, Szabo V, Dean C . Integration of CAPS markers into the RFLP map generated using recombinant inbred lines of Arabidopsis thaliana. Plant Mol Biol. 1994; 24(4):685-7. DOI: 10.1007/BF00023565. View

12.

Mindrinos M, Katagiri F, Yu G, Ausubel F . The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell. 1994; 78(6):1089-99. DOI: 10.1016/0092-8674(94)90282-8. View

13.

WARD E, Jen G . Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA. Plant Mol Biol. 1990; 14(4):561-8. DOI: 10.1007/BF00027501. View

14.

Mauch-Mani B, Slusarenko A . Arabidopsis as a model host for studying plant-pathogen interactions. Trends Microbiol. 1993; 1(7):265-70. DOI: 10.1016/0966-842x(93)90049-w. View

15.

Lander E, Green P, ABRAHAMSON J, Barlow A, Daly M, Lincoln S . MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987; 1(2):174-81. DOI: 10.1016/0888-7543(87)90010-3. View