Pollen Markers for Gene-centromere Mapping in Diploid Potato

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Oct 29

PMID 24162478

Citations 6

Authors

H J Bastiaanssen

M S Ramanna

Z Sawor

A Mincione

A V Steen

E Jacobsen

Affiliations

Soon will be listed here.

Abstract

The utility of two pollen genetic markers for estimating the extent of meiotic recombination between the centromere and a marker gene was tested in 2n pollen of diploid potato clones. One of these markers was the distal locus amylose-free (amf) on chromosome 8 and the other was the isozyme locus alcohol dehydrogenase (Adh-1) on chromosome 4. In the case of the amf locus, the gene-centromere distance was estimated in a normal synaptic and a desynaptic genotype. In both cases the genetic analysis was confined to: (1) a direct estimation of the phenotypic (blue vs red) segregation ratios in FDR (first-division restitution) 2n pollen and (2) a classification of the 4 x progeny from 4x (nulliplex amf) x 2x (Amf/amf) crosses into duplex, simplex and nulliplex classes. The recombination frequency between the centromere and the amf locus in the normal synaptic genotype B92-7015-4 corresponded to a gene-centromere distance of 48.8 cM, whereas this distance amounted to 13.3 cM in the desynaptic genotype RS93-8025-1. Hence desynapsis reduced crossing-over by 73%. The observed genetic distance of 48.8 cM in the normal synaptic clone, B92-7015-4, is the highest gene-centromere distance reported so far in potato and this could be explained on the assumption of absolute chiasma interference. For the Adh-1 locus, it was found that heterozygous 2n pollen grains could be detected in pollen samples of the diploid clones, because of the occurrence of a heterodimeric band of the isozyme. Unlike the amf locus, the genecentromere distance for the Adh-1 locus was estimated only on the basis of the duplex, simplex and nulliplex classes in the progenies from 4x (nulliplex Adh-1 (2) )x B92-7015-4 (Adh-1 (1) /Adh-1 (2) )crosses and was found to be 19.4 cM. Because the accurate positions of centromeres in relation to other loci are not available in the existing genetic maps of potato, which are saturated with molecular markers, halftetrad analysis is a promising additional approach to the basic genetics of this crop.

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References

Johnson S, Gates M, Johnson M, Talbot W, Horne S, Baik K . Centromere-linkage analysis and consolidation of the zebrafish genetic map. Genetics. 1996; 142(4):1277-88. PMC: 1207124. DOI: 10.1093/genetics/142.4.1277. View

Eppig J, Eicher E . Application of the ovarian teratoma mapping method in the mouse. Genetics. 1983; 103(4):797-812. PMC: 1202055. DOI: 10.1093/genetics/103.4.797. View

Hutten R, Schippers M, Hermsen J, Ramanna M . Comparative performance of FDR and SDR progenies from reciprocal 4x-2x crosses in potato. Theor Appl Genet. 2013; 89(5):545-50. DOI: 10.1007/BF00222446. View

Thorgaard G, Allendorf F, Knudsen K . Gene-Centromere Mapping in Rainbow Trout: High Interference over Long Map Distances. Genetics. 1983; 103(4):771-83. PMC: 1202053. DOI: 10.1093/genetics/103.4.771. View

Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U . RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet. 2013; 83(1):49-57. DOI: 10.1007/BF00229225. View

Frary A, Presting G, Tanksley S . Molecular mapping of the centromeres of tomato chromosomes 7 and 9. Mol Gen Genet. 1996; 250(3):295-304. DOI: 10.1007/BF02174387. View

Seeb J, Seeb L . Gene mapping of isozyme loci in chum salmon. J Hered. 1986; 77(6):399-402. DOI: 10.1093/oxfordjournals.jhered.a110269. View

Sherman J, Stack S . Two-dimensional spreads of synaptonemal complexes from solanaceous plants. VI. High-resolution recombination nodule map for tomato (Lycopersicon esculentum). Genetics. 1995; 141(2):683-708. PMC: 1206766. DOI: 10.1093/genetics/141.2.683. View

Tanksley S, Zamir D, Rick C . Evidence for Extensive Overlap of Sporophytic and Gametophytic Gene Expression in Lycopersicon esculentum. Science. 1981; 213(4506):453-5. DOI: 10.1126/science.213.4506.453. View

10.

Wisman E, Koornneef M, Chase T, Lifshytz E, Ramanna M, Zabel P . Genetic and molecular characterization of an Adh-1 null mutant in tomato. Mol Gen Genet. 1991; 226(1-2):120-8. DOI: 10.1007/BF00273595. View

11.

Jongedijk E, Ramanna M, Sawor Z, Hermsen J . Formation of first division restitution (FDR) 2n-megaspores through pseudohomotypic division in ds-1 (desynapsis) mutants of diploid potato: routine production of tetraploid progeny from 2xFDR × 2xFDR crosses. Theor Appl Genet. 2013; 82(5):645-56. DOI: 10.1007/BF00226804. View

12.

Qu L, Hancock J . Nature of 2n gamete formation and mode of inheritance in interspecific hybrids of diploid Vaccinium darrowi and tetraploid V. corymbosum. Theor Appl Genet. 2013; 91(8):1309-15. DOI: 10.1007/BF00220946. View

13.

Barone A, Gebhardt C, Frusciante L . Heterozygosity in 2n gametes of potato evaluated by RFLP markers. Theor Appl Genet. 2013; 91(1):98-104. DOI: 10.1007/BF00220864. View

14.

Mendiburu A, Peloquin S . Gene-centromere mapping by 4x-2x matings in potatoes. Theor Appl Genet. 2013; 54(4):177-80. DOI: 10.1007/BF00263048. View

15.

Tanksley S, Ganal M, Prince J, de Vicente M, Bonierbale M, Broun P . High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992; 132(4):1141-60. PMC: 1205235. DOI: 10.1093/genetics/132.4.1141. View

16.

Jarrell V, Lewin H, Da Y, Wheeler M . Gene-centromere mapping of bovine DYA, DRB3, and PRL using secondary oocytes and first polar bodies: evidence for four-strand double crossovers between DYA and DRB3. Genomics. 1995; 27(1):33-9. DOI: 10.1006/geno.1995.1005. View

17.

Jacobs J, van Eck H, Arens P, Verkerk-Bakker B, Te Lintel Hekkert B, Bastiaanssen H . A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers. Theor Appl Genet. 2013; 91(2):289-300. DOI: 10.1007/BF00220891. View

18.

Nel P . Crossing over and Diploid Egg Formation in the Elongate Mutant of Maize. Genetics. 1975; 79(3):435-50. PMC: 1213283. DOI: 10.1093/genetics/79.3.435. View

19.

Bonierbale M, Plaisted R, Tanksley S . RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato. Genetics. 1988; 120(4):1095-103. PMC: 1203572. DOI: 10.1093/genetics/120.4.1095. View

20.

Flipse E, Keetels C, Jacobsen E, Visser R . The dosage effect of the wildtype GBSS allele is linear for GBSS activity but not for amylose content: absence of amylose has a distinct influence on the physico-chemical properties of starch. Theor Appl Genet. 2013; 92(1):121-7. DOI: 10.1007/BF00222961. View