Electroacupuncture at 2/100 hz Activates Antinociceptive Spinal Mechanisms Different from Those Activated by Electroacupuncture at 2 and 100 hz in Responder Rats

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2013 Oct 26

PMID 24159340

Citations 8

Authors

Josie Resende Torres da Silva

Marcelo Lourenco da Silva

Wiliam Alves Prado

Affiliations

Soon will be listed here.

Abstract

We examined the effects of intrathecal injection of desipramine and fluoxetine (selective inhibitors of norepinephrine and 5-HT uptake, resp.), thiorphan and neostigmine (inhibitors of enkephalinase and acetylcholinesterase, resp.), gabapentin (a GABA releaser), and vigabatrin (an inhibitor of GABA-transaminase) on the antinociception induced by 2 Hz, 100 Hz, or 2/100 Hz electroacupuncture (EA) applied bilaterally to the Zusanli (ST36) and Sanyinjiao (SP6) acupoints using the rat tail-flick test. We show that 2 Hz EA antinociception lasts longer after the administration of drugs that increase the spinal availability of norepinephrine, acetylcholine, or GABA; 100 Hz EA antinociception lasts longer after drug that increases the spinal availability of norepinephrine; 2/100 Hz EA antinociception lasts longer after drugs that increase the spinal availability of endogenous opioids or GABA. We conclude that the antinociceptive effect of 2/100 Hz EA is different from the synergistic effect of alternate stimulation at 2 and 100 Hz because the effect of the former is not changed by increasing the spinal availability of serotonin and lasts longer after the administration of vigabatrin. The combination of EA with drugs that increase the availability of spinal neurotransmitters involved in the modulation of nociceptive inputs may result in a synergistic antinociceptive effect in the rat tail-flick test.

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References

Cheng J, Lai Y, Chen C, Cheng C, Chiou L . Magnesium chloride and ruthenium red attenuate the antiallodynic effect of intrathecal gabapentin in a rat model of postoperative pain. Anesthesiology. 2003; 98(6):1472-9. DOI: 10.1097/00000542-200306000-00026. View

Shen E, Tsai T, Lan C . Supraspinal participation in the inhibitory effect of acupuncture on viscero-somatic reflex discharges. Chin Med J (Engl). 1975; 1(6):431-40. View

Han J, Re M, Tang J, Fan S, Xu J, Guan X . The role of central catecholamine in acupuncture analgesia. Chin Med J (Engl). 1979; 92(11):793-800. View

Huang C, Wang Y, Han J, Wan Y . Characteristics of electroacupuncture-induced analgesia in mice: variation with strain, frequency, intensity and opioid involvement. Brain Res. 2002; 945(1):20-5. DOI: 10.1016/s0006-8993(02)02503-9. View

Gassner M, Ruscheweyh R, Sandkuhler J . Direct excitation of spinal GABAergic interneurons by noradrenaline. Pain. 2009; 145(1-2):204-10. DOI: 10.1016/j.pain.2009.06.021. View

Yoon M, Choi J, Jeong S . Antinociception of intrathecal cholinesterase inhibitors and cholinergic receptors in rats. Acta Anaesthesiol Scand. 2003; 47(9):1079-84. DOI: 10.1034/j.1399-6576.2003.00212.x. View

Xing G, Liu F, Qu X, Han J, Wan Y . Long-term synaptic plasticity in the spinal dorsal horn and its modulation by electroacupuncture in rats with neuropathic pain. Exp Neurol. 2007; 208(2):323-32. DOI: 10.1016/j.expneurol.2007.09.004. View

Han J, Terenius L . Neurochemical basis of acupuncture analgesia. Annu Rev Pharmacol Toxicol. 1982; 22:193-220. DOI: 10.1146/annurev.pa.22.040182.001205. View

Cheng J, Chiou L . Mechanisms of the antinociceptive action of gabapentin. J Pharmacol Sci. 2006; 100(5):471-86. DOI: 10.1254/jphs.cr0050020. View

10.

Li W, Cui K, Li N, Gu Q, Schwarz W, Ding G . Analgesic effect of electroacupuncture on complete Freund's adjuvant-induced inflammatory pain in mice: a model of antipain treatment by acupuncture in mice. Jpn J Physiol. 2005; 55(6):339-44. DOI: 10.2170/jjphysiol.RP001505. View

11.

Beal B, Moeller-Bertram T, Schilling J, Wallace M . Gabapentin for once-daily treatment of post-herpetic neuralgia: a review. Clin Interv Aging. 2012; 7:249-55. PMC: 3410680. DOI: 10.2147/CIA.S23783. View

12.

Niu C, Hao H, Lu J, Li L, Han Z, Tu Y . A novel uni-acupoint electroacupuncture stimulation method for pain relief. Evid Based Complement Alternat Med. 2009; 2011:209879. PMC: 3095408. DOI: 10.1093/ecam/nep104. View

13.

Stark P, Fuller R, Wong D . The pharmacologic profile of fluoxetine. J Clin Psychiatry. 1985; 46(3 Pt 2):7-13. View

14.

Xu W, Qiu X, Han J . Serotonin receptor subtypes in spinal antinociception in the rat. J Pharmacol Exp Ther. 1994; 269(3):1182-9. View

15.

Sudakov S, Borisova E, Lyupina Y . Influence of inheritance and fostering on sensitivity to effects of morphine on nociception and locomotor activity in two inbred rat strains. Neuropharmacology. 1996; 35(8):1131-4. DOI: 10.1016/s0028-3908(96)00042-1. View

16.

Petroff O, Rothman D . Measuring human brain GABA in vivo: effects of GABA-transaminase inhibition with vigabatrin. Mol Neurobiol. 1998; 16(1):97-121. DOI: 10.1007/BF02740605. View

17.

Hwang S, Wilcox G . Analgesic properties of intrathecally administered heterocyclic antidepressants. Pain. 1987; 28(3):343-355. DOI: 10.1016/0304-3959(87)90068-6. View

18.

Malseed R, GOLDSTEIN F . Enhancement of morphine analgesia by tricyclic antidepressants. Neuropharmacology. 1979; 18(10):827-9. DOI: 10.1016/0028-3908(79)90028-5. View

19.

Larson A, TAKEMORI A . Effect of fluoxetine hydrochloride (Lilly 110140), a specific inhibitor of serotonin uptake, on morphine analgesia and the development of tolerance. Life Sci. 1977; 21(12):1807-11. DOI: 10.1016/0024-3205(77)90162-x. View

20.

Millan M . Descending control of pain. Prog Neurobiol. 2002; 66(6):355-474. DOI: 10.1016/s0301-0082(02)00009-6. View