Manifestations of Fasting-induced Fatty Liver and Rapid Recovery from Steatosis in Voles Fed Lard or Flaxseed Oil Lipids

Overview

Journal Nutrients

Date 2013 Oct 25

PMID 24152753

Citations 5

Authors

Anne-Mari Mustonen

Vesa Karja

Michael Kilpio

Raija Tammi

Markku Tammi

Kirsti Rouvinen-Watt

Toivo Halonen

Petteri Nieminen

Affiliations

Soon will be listed here.

Abstract

Long-chain n-3 polyunsaturated fatty acids (PUFA) can have beneficial effects against fat deposition, cardiovascular diseases, and liver steatosis. We investigated how diets based on lard (predominantly saturated and monounsaturated fatty acids) or flaxseed oil (rich in 18:3n-3) affect liver fat-% and fatty acid profiles of tundra voles (Microtus oeconomus). We also studied potential participation of hyaluronan (HA) in the pathology of fatty liver and whether the development and recovery of fasting-induced steatosis are influenced by n-3 PUFA. The dietary fatty acid composition was manifested in the liver fatty acid signatures. Fasting for 18 h induced macrovesicular steatosis and the liver fat-% increased to 22% independent of the preceding diet. Fasting-induced steatosis did not involve inflammation or connective tissue activation indicated by the absence of both leukocyte accumulation and increased HA. Food deprivation modified the liver fatty acid signatures to resemble more closely the diets. Fasting reduced the proportions of long-chain n-3 PUFA in both dietary regimes and n-3/n-6 PUFA ratios in the lard-fed voles. Decreases in long-chain n-3 PUFA may promote lipid accumulation by modulating the expression of lipid-metabolizing genes. Dietary 18:3n-3 did not prevent the development or attenuate the manifestation of steatosis in the fasted voles or promote the recovery.

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References

Kirpich I, Gobejishvili L, Bon Homme M, Waigel S, Cave M, Arteel G . Integrated hepatic transcriptome and proteome analysis of mice with high-fat diet-induced nonalcoholic fatty liver disease. J Nutr Biochem. 2010; 22(1):38-45. PMC: 3860361. DOI: 10.1016/j.jnutbio.2009.11.009. View

Montazeri G, Estakhri A, Mohamadnejad M, Nouri N, Montazeri F, Mohammadkani A . Serum hyaluronate as a non-invasive marker of hepatic fibrosis and inflammation in HBeAg-negative chronic hepatitis B. BMC Gastroenterol. 2005; 5:32. PMC: 1266362. DOI: 10.1186/1471-230X-5-32. View

Clarke S . Polyunsaturated fatty acid regulation of gene transcription: a molecular mechanism to improve the metabolic syndrome. J Nutr. 2001; 131(4):1129-32. DOI: 10.1093/jn/131.4.1129. View

Shapiro H, Tehilla M, Attal-Singer J, Bruck R, Luzzatti R, Singer P . The therapeutic potential of long-chain omega-3 fatty acids in nonalcoholic fatty liver disease. Clin Nutr. 2010; 30(1):6-19. DOI: 10.1016/j.clnu.2010.06.001. View

Bloedon L, Szapary P . Flaxseed and cardiovascular risk. Nutr Rev. 2004; 62(1):18-27. DOI: 10.1111/j.1753-4887.2004.tb00002.x. View

Sharma S, Barrett F, Adamson J, Todd A, Megson I, Zentler-Munro P . Diabetic fatty liver disease is associated with specific changes in blood-borne markers. Diabetes Metab Res Rev. 2012; 28(4):343-8. DOI: 10.1002/dmrr.2269. View

Capanni M, Calella F, Biagini M, Genise S, Raimondi L, Bedogni G . Prolonged n-3 polyunsaturated fatty acid supplementation ameliorates hepatic steatosis in patients with non-alcoholic fatty liver disease: a pilot study. Aliment Pharmacol Ther. 2006; 23(8):1143-51. DOI: 10.1111/j.1365-2036.2006.02885.x. View

Dieterich R, PRESTON D . Atherosclerosis in lemmings and voles fed a high fat, high cholesterol diet. Atherosclerosis. 1979; 33(2):181-9. DOI: 10.1016/0021-9150(79)90115-1. View

Fraulob J, Ogg-Diamantino R, Fernandes-Santos C, Aguila M, Mandarim-de-Lacerda C . A Mouse Model of Metabolic Syndrome: Insulin Resistance, Fatty Liver and Non-Alcoholic Fatty Pancreas Disease (NAFPD) in C57BL/6 Mice Fed a High Fat Diet. J Clin Biochem Nutr. 2010; 46(3):212-23. PMC: 2872226. DOI: 10.3164/jcbn.09-83. View

10.

Kajikawa S, Harada T, Kawashima A, Imada K, Mizuguchi K . Highly purified eicosapentaenoic acid prevents the progression of hepatic steatosis by repressing monounsaturated fatty acid synthesis in high-fat/high-sucrose diet-fed mice. Prostaglandins Leukot Essent Fatty Acids. 2009; 80(4):229-38. DOI: 10.1016/j.plefa.2009.02.004. View

11.

Larsson S, Kumlin M, Ingelman-Sundberg M, Wolk A . Dietary long-chain n-3 fatty acids for the prevention of cancer: a review of potential mechanisms. Am J Clin Nutr. 2004; 79(6):935-45. DOI: 10.1093/ajcn/79.6.935. View

12.

Jung U, Millman P, Tall A, Deckelbaum R . n-3 fatty acids ameliorate hepatic steatosis and dysfunction after LXR agonist ingestion in mice. Biochim Biophys Acta. 2011; 1811(9):491-7. PMC: 3196345. DOI: 10.1016/j.bbalip.2011.06.003. View

13.

Carter-Kent C, Brunt E, Yerian L, Alkhouri N, Angulo P, Kohli R . Relations of steatosis type, grade, and zonality to histological features in pediatric nonalcoholic fatty liver disease. J Pediatr Gastroenterol Nutr. 2011; 52(2):190-7. DOI: 10.1097/MPG.0b013e3181fb47d3. View

14.

Febel H, Mezes M, Palfy T, Herman A, Gundel J, Lugasi A . Effect of dietary fatty acid pattern on growth, body fat composition and antioxidant parameters in broilers. J Anim Physiol Anim Nutr (Berl). 2008; 92(3):369-76. DOI: 10.1111/j.1439-0396.2008.00803.x. View

15.

Puri P, Baillie R, Wiest M, Mirshahi F, Choudhury J, Cheung O . A lipidomic analysis of nonalcoholic fatty liver disease. Hepatology. 2007; 46(4):1081-90. DOI: 10.1002/hep.21763. View

16.

Kleiner D, Brunt E, Van Natta M, Behling C, Contos M, Cummings O . Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology. 2005; 41(6):1313-21. DOI: 10.1002/hep.20701. View

17.

Tammi R, Pasonen-Seppanen S, Kolehmainen E, Tammi M . Hyaluronan synthase induction and hyaluronan accumulation in mouse epidermis following skin injury. J Invest Dermatol. 2005; 124(5):898-905. DOI: 10.1111/j.0022-202X.2005.23697.x. View

18.

Cortez-Pinto H, Jesus L, Barros H, Lopes C, Moura M, Camilo M . How different is the dietary pattern in non-alcoholic steatohepatitis patients?. Clin Nutr. 2006; 25(5):816-23. DOI: 10.1016/j.clnu.2006.01.027. View

19.

Alwayn I, Gura K, Nose V, Zausche B, Javid P, Garza J . Omega-3 fatty acid supplementation prevents hepatic steatosis in a murine model of nonalcoholic fatty liver disease. Pediatr Res. 2005; 57(3):445-52. DOI: 10.1203/01.PDR.0000153672.43030.75. View

20.

Ichida T, Sugitani S, Satoh T, Matsuda Y, Sugiyama M, Yonekura K . Localization of hyaluronan in human liver sinusoids: a histochemical study using hyaluronan-binding protein. Liver. 1996; 16(6):365-71. DOI: 10.1111/j.1600-0676.1996.tb00763.x. View