Bidirectional Transcription of Maize Streak Virus DNA and Identification of the Coat Protein Gene

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1985 Oct 25

PMID 2414736

Citations 30

Authors

B A Morris-Krsinich

P M Mullineaux

J Donson

M I Boulton

P G Markham

M N Short

J W Davies

Affiliations

Soon will be listed here.

Abstract

Three RNA transcripts encoded by maize streak virus DNA were detected in polyadenylated RNA from virus-infected maize leaves. Two of the transcripts, a major 0.9 kb and a minor 1.05 kb RNA, were mapped on the virion (+) sense DNA and the other minor transcript of 1.2 kb was mapped on the complementary (-) sense DNA, demonstrating that transcription of MSV DNA was bidirectional. The two virion sense transcripts were 3' coterminal at nucleotide 1114 but had 5' termini at nucleotides 2682 and 163 respectively. Virus-specific polyadenylated RNA translated in vitro to produce a 28,000 MW polypeptide, specifically immunoprecipitable by antiserum raised against whole virus. The mRNA for this protein was mapped by hybrid-arrested translation to the long open reading frame in virion sense DNA whose potential amino acid composition, calculated from nucleotide sequence data, closely agreed with that determined experimentally for the coat protein.

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References

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Howarth A, Caton J, Bossert M, Goodman R . Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proc Natl Acad Sci U S A. 1985; 82(11):3572-6. PMC: 397827. DOI: 10.1073/pnas.82.11.3572. View

Berk A, Sharp P . Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977; 12(3):721-32. DOI: 10.1016/0092-8674(77)90272-0. View

Hamilton W, Stein V, Coutts R, Buck K . Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO J. 1984; 3(9):2197-205. PMC: 557666. DOI: 10.1002/j.1460-2075.1984.tb02114.x. View

Townsend R, Stanley J, Curson S, Short M . Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein. EMBO J. 1985; 4(1):33-7. PMC: 554147. DOI: 10.1002/j.1460-2075.1985.tb02313.x. View

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

Rees M . The estimation of threonine and serine in proteins. Biochem J. 1946; 40(5-6):632-40. PMC: 1270019. DOI: 10.1042/bj0400632. View

Mullineaux P, Donson J, Morris-Krsinich B, Boulton M, Davies J . The nucleotide sequence of maize streak virus DNA. EMBO J. 1984; 3(13):3063-8. PMC: 557817. DOI: 10.1002/j.1460-2075.1984.tb02258.x. View

10.

Gay N, Walker J . Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase. Nature. 1983; 301(5897):262-4. DOI: 10.1038/301262a0. View

11.

Donson J, Morris-Krsinich B, Mullineaux P, Boulton M, Davies J . A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated. EMBO J. 1984; 3(13):3069-73. PMC: 557819. DOI: 10.1002/j.1460-2075.1984.tb02260.x. View

12.

Penke B, Ferenczi R, Kovacs K . A new acid hydrolysis method for determining tryptophan in peptides and proteins. Anal Biochem. 1974; 60(1):45-50. DOI: 10.1016/0003-2697(74)90129-8. View

13.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

14.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

15.

Pelham H, Jackson R . An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976; 67(1):247-56. DOI: 10.1111/j.1432-1033.1976.tb10656.x. View

16.

Kozak M . Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984; 12(2):857-72. PMC: 318541. DOI: 10.1093/nar/12.2.857. View

17.

Weaver R, Weissmann C . Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res. 1979; 7(5):1175-93. PMC: 342295. DOI: 10.1093/nar/7.5.1175. View

18.

Laskey R, Mills A . Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975; 56(2):335-41. DOI: 10.1111/j.1432-1033.1975.tb02238.x. View

19.

Favaloro J, Treisman R, Kamen R . Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol. 1980; 65(1):718-49. DOI: 10.1016/s0076-6879(80)65070-8. View

20.

Hull R, Davies J . Genetic engineering with plant viruses, and their potential as vectors. Adv Virus Res. 1983; 28:1-33. DOI: 10.1016/s0065-3527(08)60720-4. View