Voltage-gated Ca2+ Current in Pancreatic B-cells

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1985 Aug 1

PMID 2414720

Citations 49

Authors

L S Satin

D L Cook

Affiliations

Soon will be listed here.

Abstract

Voltage-dependent inward Ba++ and Ca++ currents were recorded in cultured neonatal rat pancreatic islet cells using the whole-cell voltage clamp technique. Outward current was suppressed by internal Cs+ and ATP and external TEA. Inward currents activated rapidly and decayed to a variable extent. The current decay was particularly marked when using long duration or large depolarizing pulses. Currents were due to Ca++ channel activation since they were abolished by omitting Ba++ and Ca++ or including Co++.

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References

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Cook D, CRILL W, Porte Jr D . Plateau potentials in pancreatic islet cells are voltage-dependent action potentials. Nature. 1980; 286(5771):404-6. DOI: 10.1038/286404a0. View

Fujimoto W, Metz S . Phasic glucose-stimulated insulin secretion by neonatal rat pancreatic islet cells. Enhancement by sodium salicylate. Diabetes. 1984; 33(9):872-8. DOI: 10.2337/diab.33.9.872. View

Fenwick E, Marty A, Neher E . Sodium and calcium channels in bovine chromaffin cells. J Physiol. 1982; 331:599-635. PMC: 1197771. DOI: 10.1113/jphysiol.1982.sp014394. View

Cook D, Hales C . Intracellular ATP directly blocks K+ channels in pancreatic B-cells. Nature. 1984; 311(5983):271-3. DOI: 10.1038/311271a0. View

DEAN P, Matthews E . Electrical activity in pancreatic islet cells: effect of ions. J Physiol. 1970; 210(2):265-75. PMC: 1395563. DOI: 10.1113/jphysiol.1970.sp009208. View

Hagiwara S, Byerly L . Calcium channel. Annu Rev Neurosci. 1981; 4:69-125. DOI: 10.1146/annurev.ne.04.030181.000441. View

Cook D . Electrical pacemaker mechanisms of pancreatic islet cells. Fed Proc. 1984; 43(9):2368-72. View

Ribalet B, BEIGELMAN P . Calcium action potentials and potassium permeability activation in pancreatic beta-cells. Am J Physiol. 1980; 239(3):C124-33. DOI: 10.1152/ajpcell.1980.239.3.C124. View

10.

Wollheim C, SHARP G . Regulation of insulin release by calcium. Physiol Rev. 1981; 61(4):914-73. DOI: 10.1152/physrev.1981.61.4.914. View

11.

Cook D, Ikeuchi M, Fujimoto W . Lowering of pHi inhibits Ca2+-activated K+ channels in pancreatic B-cells. Nature. 1984; 311(5983):269-71. DOI: 10.1038/311269a0. View

12.

Fujimoto W . Purification of neonatal rat pancreatic monolayer cultures for endocrine cells. Proc Soc Exp Biol Med. 1979; 162(1):241-4. DOI: 10.3181/00379727-162-40657. View

13.

Ikeuchi M, Yagi K . Pancreatic A cell generates action potential. Jpn J Physiol. 1982; 32(5):873-8. DOI: 10.2170/jjphysiol.32.873. View