Antibacterial Effects of Apis Mellifera and Stingless Bees Honeys on Susceptible and Resistant Strains of Escherichia Coli, Staphylococcus Aureus and Klebsiella Pneumoniae in Gondar, Northwest Ethiopia

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2013 Oct 22

PMID 24138782

Citations 18

Authors

Yalemwork Ewnetu

Wossenseged Lemma

Nega Birhane

Affiliations

Soon will be listed here.

Abstract

Background: Honey is a natural substance produced by honeybees and has nutritional and therapeutic uses. In Ethiopia, honeys are used traditionally to treat wounds, respiratory infections and diarrhoea. Recent increase of drug resistant bacteria against the existing antibiotics forced investigators to search for alternative natural remedies and evaluate their potential use on scientific bases. Thus, the aim of this study was to evaluate the antibacterial effects of different types of honeys in Ethiopia which are used traditionally to treat different types of respiratory and gastrointestinal infections.

Methods: Mueller Hinton agar (70191) diffusion and nutrient broth culture medium assays were performed to determine susceptibility of Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922) and resistant clinical isolates (Methicillin resistant Staphylococcus aureus(MRSA), Escherichia coli(R) and Klebsiella pneumoniae (R), using honeys of Apis mellifera and stingless bees in northern and north western Ethiopia.

Results: Honey of the stingless bees produced the highest mean inhibition (22.27 ± 3.79 mm) compared to white honey (21.0 ± 2.7 mm) and yellow honey (18.0 ± 2.3 mm) at 50% (v/v) concentration on all the standard and resistant strains. Stingless bees honey was found to have Minimum Inhibitory Concentration (MIC) of 6.25% (6.25 mg/ml) for 80% of the test organisms compared to 40% for white and yellow Apis mellifera honeys. All the honeys were found to have minimum bactericidal concentration (MBC) of 12.5% (12.5 mg/ml) against all the test organisms. Staphylococcus aureus (ATCC 25923) was susceptible to amoxicillin, methicillin, kanamycine, tetracycline, and vancomycine standard antibiotic discs used for susceptibility tests. Similarly, Escherichia coli (ATCC 25922) was found susceptible for kanamycine, tetracycline and vancomycine. Escherichia coli (ATCC 25922) has not been tested for amoxicillin ampicillin and methicillin. The susceptibility tests performed against Staphylococcus aureus (MRSA), Escherichia coli (R) and Klebsiella pneumoniae (R) using three of methicillin, erythromycin, ampicillin, Penicillin and amoxicillin discs were resistant. But, these drug resistant strains were susceptible to antibacterial agents found in the honeys and inhibited from 16 mm to 20.33 mm.

Conclusions: Honeys in Ethiopia can be used as therapeutic agents for drug resistant bacteria after pharmaceutical standardization and clinical trials.

Citing Articles

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References

Namias N . Honey in the management of infections. Surg Infect (Larchmt). 2003; 4(2):219-26. DOI: 10.1089/109629603766957022. View

Tanih N, Dube C, Green E, Mkwetshana N, Clarke A, Ndip L . An African perspective on Helicobacter pylori: prevalence of human infection, drug resistance, and alternative approaches to treatment. Ann Trop Med Parasitol. 2009; 103(3):189-204. DOI: 10.1179/136485909X398311. View

Gheldof N, Wang X, Engeseth N . Identification and quantification of antioxidant components of honeys from various floral sources. J Agric Food Chem. 2002; 50(21):5870-7. DOI: 10.1021/jf0256135. View

Temaru E, Shimura S, Amano K, Karasawa T . Antibacterial activity of honey from stingless honeybees (Hymenoptera; Apidae; Meliponinae). Pol J Microbiol. 2008; 56(4):281-5. View

Fahey J, Stephenson K . Pinostrobin from honey and Thai ginger (Boesenbergia pandurata): a potent flavonoid inducer of mammalian phase 2 chemoprotective and antioxidant enzymes. J Agric Food Chem. 2002; 50(25):7472-6. DOI: 10.1021/jf025692k. View

Farnesi A, Aquino-Ferreira R, de Jong D, Bastos J, Soares A . Effects of stingless bee and honey bee propolis on four species of bacteria. Genet Mol Res. 2009; 8(2):635-40. DOI: 10.4238/vol8-2kerr023. View

Burdock G . Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol. 1998; 36(4):347-63. DOI: 10.1016/s0278-6915(97)00145-2. View

Sato T, Miyata G . The nutraceutical benefit, part iii: honey. Nutrition. 2000; 16(6):468-9. DOI: 10.1016/s0899-9007(00)00271-9. View

Taormina P, Niemira B, Beuchat L . Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. Int J Food Microbiol. 2001; 69(3):217-25. DOI: 10.1016/s0168-1605(01)00505-0. View

10.

Allen K, Molan P, Reid G . A survey of the antibacterial activity of some New Zealand honeys. J Pharm Pharmacol. 1991; 43(12):817-22. DOI: 10.1111/j.2042-7158.1991.tb03186.x. View

11.

Ndip R, Malange Takang A, Echakachi C, Malongue A, Akoachere J, Ndip L . In-vitro antimicrobial activity of selected honeys on clinical isolates of Helicobacter pylori. Afr Health Sci. 2011; 7(4):228-32. PMC: 3074369. View

12.

Dunford C, Cooper R, Molan P, White R . The use of honey in wound management. Nurs Stand. 2002; 15(11):63-8. DOI: 10.7748/ns2000.11.15.11.63.c2952. View

13.

Andrews J, Wise R . Susceptibility testing of Bacillus species. J Antimicrob Chemother. 2002; 49(6):1040-2. DOI: 10.1093/jac/dkf063. View

14.

Iurlina M, Fritz R . Characterization of microorganisms in Argentinean honeys from different sources. Int J Food Microbiol. 2005; 105(3):297-304. DOI: 10.1016/j.ijfoodmicro.2005.03.017. View

15.

Cooper R, Molan P, Harding K . Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds. J R Soc Med. 1999; 92(6):283-5. PMC: 1297205. DOI: 10.1177/014107689909200604. View

16.

Andualem B . Combined antibacterial activity of stingless bee (Apis mellipodae) honey and garlic (Allium sativum) extracts against standard and clinical pathogenic bacteria. Asian Pac J Trop Biomed. 2013; 3(9):725-31. PMC: 3757282. DOI: 10.1016/S2221-1691(13)60146-X. View

17.

Bansal V, Medhi B, Pandhi P . Honey--a remedy rediscovered and its therapeutic utility. Kathmandu Univ Med J (KUMJ). 2008; 3(3):305-9. View

18.

Willix D, Molan P, Harfoot C . A comparison of the sensitivity of wound-infecting species of bacteria to the antibacterial activity of manuka honey and other honey. J Appl Bacteriol. 1992; 73(5):388-94. DOI: 10.1111/j.1365-2672.1992.tb04993.x. View

19.

Tonks A, Dudley E, Porter N, Parton J, Brazier J, Smith E . A 5.8-kDa component of manuka honey stimulates immune cells via TLR4. J Leukoc Biol. 2007; 82(5):1147-55. DOI: 10.1189/jlb.1106683. View

20.

Gheldof N, Wang X, Engeseth N . Buckwheat honey increases serum antioxidant capacity in humans. J Agric Food Chem. 2003; 51(5):1500-5. DOI: 10.1021/jf025897t. View