» Articles » PMID: 24137293

Human Adipose-derived Stem Cell Adipogenesis Induces Paracrine Regulation of the Invasive Ability of MCF-7 Human Breast Cancer Cells

Overview
Journal Exp Ther Med
Specialty Pathology
Date 2013 Oct 19
PMID 24137293
Citations 9
Authors
Affiliations
Soon will be listed here.
Abstract

The aim of this study was to determine the effects of paracrine regulation on the invasive ability of MCF-7 human breast cancer cells through human adipose-derived stem cell (hADSC) adipogenesis. hADSC differentiation of the third and fourth passages of cells was induced in different induction media: osteogenic, adipogenic and chondrogenic. Transwell migration assays in the differently conditioned media, flow cytometry, enzyme-linked immunosorbent assay and western blot analysis for selected cytokines were performed. The flow cytometric analysis demonstrated positive expression of CD29, CD44 and CD105, while expression of CD34 and CD45 was not identified. The transwell migration assay showed that the invasive ability of MCF-7 cells was significantly enhanced during hADSC adipogenesis. hADSCs exerted a significantly positive effect on the invasive activity of MCF-7 cells during adipo-genesis. The results indicate that the high expression levels of activating protein 2 (aP2) in MCF-7 and adipocytes induced for 12 days may be associated with cell growth, invasion and metastasis. Peroxisome proliferator-activated receptor γ may be involved in fatty syntheses during adipogenic initiation and following adipogenic differentiation, possibly acting as a protection factor resulting in cell maturation and differentiation. This study also demonstrated that the expression of vascular endothelial growth factor was repressed by hADSCs, while that of matrix metalloproteinase-2 and urokinase-type plasminogen activator was increased to a significant level.

Citing Articles

Highly Concentrated Stabilized Hybrid Complexes of Hyaluronic Acid: Rheological and Biological Assessment of Compatibility with Adipose Tissue and Derived Stromal Cells towards Regenerative Medicine.

Vassallo V, Di Meo C, Alessio N, La Gatta A, Ferraro G, Nicoletti G Int J Mol Sci. 2024; 25(4).

PMID: 38396698 PMC: 10888561. DOI: 10.3390/ijms25042019.


Netrin-1 promotes the vasculogenic capacity of human adipose-derived stem cells.

Luo M, Zhu J, Gao X Cell Tissue Bank. 2022; 24(2):357-367.

PMID: 36222969 DOI: 10.1007/s10561-022-10038-0.


Mesenchymal Stem Cell Application and Its Therapeutic Mechanisms in Intracerebral Hemorrhage.

Yang G, Fan X, Mazhar M, Yang S, Xu H, Dechsupa N Front Cell Neurosci. 2022; 16:898497.

PMID: 35769327 PMC: 9234141. DOI: 10.3389/fncel.2022.898497.


Mechanism of White Matter Injury and Promising Therapeutic Strategies of MSCs After Intracerebral Hemorrhage.

Li J, Xiao L, He D, Luo Y, Sun H Front Aging Neurosci. 2021; 13:632054.

PMID: 33927608 PMC: 8078548. DOI: 10.3389/fnagi.2021.632054.


Degradation of selenoprotein S and selenoprotein K through PPARγ-mediated ubiquitination is required for adipocyte differentiation.

Lee J, Jang J, Ko K, Jin Y, Ham M, Kang H Cell Death Differ. 2018; 26(6):1007-1023.

PMID: 30082770 PMC: 6748118. DOI: 10.1038/s41418-018-0180-x.


References
1.
Moldes M, Zuo Y, Morrison R, Silva D, Park B, Liu J . Peroxisome-proliferator-activated receptor gamma suppresses Wnt/beta-catenin signalling during adipogenesis. Biochem J. 2003; 376(Pt 3):607-13. PMC: 1223802. DOI: 10.1042/BJ20030426. View

2.
Tang L, Han X . The urokinase plasminogen activator system in breast cancer invasion and metastasis. Biomed Pharmacother. 2012; 67(2):179-82. DOI: 10.1016/j.biopha.2012.10.003. View

3.
Wang X, Zhou Q, Du J, Zhang H, Lu Y, Su S . Baicalin suppresses migration, invasion and metastasis of breast cancer via p38MAPK signaling pathway. Anticancer Agents Med Chem. 2013; 13(6):923-31. DOI: 10.2174/18715206113139990143. View

4.
Pellikainen J, Kosma V . Activator protein-2 in carcinogenesis with a special reference to breast cancer--a mini review. Int J Cancer. 2007; 120(10):2061-7. DOI: 10.1002/ijc.22648. View

5.
Bucky L, Percec I . The science of autologous fat grafting: views on current and future approaches to neoadipogenesis. Aesthet Surg J. 2008; 28(3):313-21. DOI: 10.1016/j.asj.2008.02.004. View