Induction of Apoptosis by Sarijang, a Bamboo Salt Sauce, in U937 Human Leukemia Cells Through the Activation of Caspases

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2013 Oct 19

PMID 24137193

Authors

Eun-A Choi

Cheol Park

Min-Ho Han

Jun Hyuk Lee

Gi-Young Kim

Byung Tae Choi

Yung Hyun Choi

Affiliations

Soon will be listed here.

Abstract

Sarijang is a bamboo salt soy sauce, containing extracts of , sulfur-fed duck, dried bark of and , which has been demonstrated to exert anti-inflammatory and antitumor activity. However, the cellular and molecular mechanisms of action of sarijang have not yet been elucidated. In the present study, we investigated the pro-apoptotic effects of sarijang in an U937 human leukemia cell model. Treatment with sarijang resulted in a concentration-dependent growth inhibition of the cells, coupled with the characteristic morphological features of apoptosis. The induction of the apoptotic cell death of the U937 cells by sarijang exhibited a correlation with the upregulation of death receptor 4 (DR4), the downregulation of members of the inhibitor of apoptosis protein (IAP) family, including survivin and cellular IAP (cIAP)-1, and the cleavage of Bid. Apoptosis-inducing concentrations of sarijang also induced the activation of caspases (caspase-3, -8 and -9), accompanied by proteolytic degradation of poly(ADP-ribose)-polymerase, β-catenin and phospholipase C-γ1. However, the apoptosis induced by sarijang was significantly inhibited by z-VED-fmk, a pan-caspase inhibitor, which demonstrated the importance of caspases in the process. These results suggested that sarijang may be a potential chemotherapeutic agent for use in the control of U937 human leukemia cells. Further studies are required to identify the active compounds in sarijang.

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