Photodamage of Lipid Bilayers by Irradiation of a Fluorescently Labeled Cell-penetrating Peptide

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2013 Oct 19

PMID 24135456

Citations 14

Authors

Igor Meerovich

Nandhini Muthukrishnan

Gregory A Johnson

Alfredo Erazo-Oliveras

Jean-Philippe Pellois

Affiliations

Soon will be listed here.

Abstract

Background: Fluorescently labeled cell-penetrating peptides can translocate into cells by endocytosis and upon light irradiation, lyse the endocytic vesicles. This photo-inducible endosomolytic activity of Fl-CPPs can be used to efficiently deliver macromolecules such as proteins and nucleic acids and other small organic molecules into the cytosol of live cells. The requirement of a light trigger to induce photolysis provides a more spatial and temporal control to the intracellular delivery process.

Methods: In this report, we examine the molecular level mechanisms by which cell-penetrating peptides such as TAT when labeled with small organic fluorophore molecules acquire a photo-induced lytic activity using a simplified model of lipid vesicles.

Results: The peptide TAT labeled with 5(6)-carboxytetramethylrhodamine binds to negatively charged phospholipids, thereby bringing the fluorophore in close proximity to the membrane of liposomes. Upon light irradiation, the excited fluorophore produces reactive oxygen species at the lipid bilayer and oxidation of the membrane is achieved. In addition, the fluorescent peptide causes aggregation of photo-oxidized lipids, an activity that requires the presence of arginine residues in the peptide sequence.

Conclusions: These results suggest that the cell-penetrating peptide plays a dual role. On one hand, TAT targets a conjugated fluorophore to membranes. On the other hand, TAT participates directly in the destabilization of photosensitized membranes. Peptide and fluorophore therefore appear to act in synergy to destroy membranes efficiently.

General Significance: Understanding the mechanism behind Fl-CPP mediated membrane photodamage will help to design optimally photo-endosomolytic compounds.

Citing Articles

Photoinduced Endosomal Escape Mechanism: A View from Photochemical Internalization Mediated by CPP-Photosensitizer Conjugates.

Soe T, Watanabe K, Ohtsuki T Molecules. 2020; 26(1).

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Cell cycle dependence of apoptosis photo-triggered using peptide-photosensitizer conjugate.

Kim H, Watanabe S, Kitamatsu M, Watanabe K, Ohtsuki T Sci Rep. 2020; 10(1):19087.

PMID: 33154435 PMC: 7644668. DOI: 10.1038/s41598-020-76100-7.

Photochemical Internalization for Intracellular Drug Delivery. From Basic Mechanisms to Clinical Research.

Jerjes W, Theodossiou T, Hirschberg H, Hogset A, Weyergang A, Selbo P J Clin Med. 2020; 9(2).

PMID: 32075165 PMC: 7073817. DOI: 10.3390/jcm9020528.

Codelivery of a cytotoxin and photosensitiser via a liposomal nanocarrier: a novel strategy for light-triggered cytosolic release.

Yaghini E, Dondi R, Edler K, Loizidou M, MacRobert A, Eggleston I Nanoscale. 2018; 10(43):20366-20376.

PMID: 30376028 PMC: 6251340. DOI: 10.1039/c8nr04048f.

Fluorescent and Photosensitizing Conjugates of Cell-Penetrating Peptide TAT(47-57): Design, Microwave-Assisted Synthesis at 60 °C, and Properties.

Okuda-Shinagawa N, Moskalenko Y, Junqueira H, Baptista M, Marques C, Machini M ACS Omega. 2018; 2(11):8156-8166.

PMID: 30023576 PMC: 6044864. DOI: 10.1021/acsomega.7b01127.

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