Controversial Effect of Ethanol Irrespective of Kinases Inhibition on the Agonist-dependant Vasoconstriction

Overview

Journal Biomol Ther (Seoul)

Specialty Pharmacology

Date 2013 Oct 17

PMID 24130935

Citations 1

Authors

Hyun Dong Je

Hyeong-Dong Kim

June Hong Park

Affiliations

Soon will be listed here.

Abstract

The present study was undertaken to determine whether ethanol influences on the agonist-induced vascular smooth muscle contraction and, if so, to investigate the related mechanism. The measurement of isometric contractions using a computerized data acquisition system was combined with molecular experiments. Ethanol significantly inhibited thromboxane A2 mimetic-induced contraction with intact endothelial function, but there was no relaxation on thromboxane A2 mimetic U-46619-induced contraction irrespective of endothelium suggesting that the pathway such as Rho-kinase activation, Ca(2+) entry or thin filament regulation was not affected. In addition, ethanol didn't decrease thromboxane A2 mimetic-induced increase of phospho-myosin phosphatase targeting subunit protein 1 (pMYPT1) or pERK1/2. Interestingly, ethanol didn't inhibit significantly phorbol ester-induced contraction in denuded muscles suggesting that thin filament regulation is less important on the ethanol-induced regulation in the muscle than endothelial NO synthesis. In conclusion, this study provides the evidence and possible related mechanism concerning the effect of ethanol on the agonist-dependent contraction in rat aortic rings with regard to endothelial function.

Citing Articles

Acute alcohol induces greater dose-dependent increase in the lateral cortical network functional connectivity in adult than adolescent rats.

Lee S, Shnitko T, Hsu L, Broadwater M, Sardinas M, Wang T Addict Neurosci. 2023; 7.

PMID: 37576436 PMC: 10421607. DOI: 10.1016/j.addicn.2023.100105.

References

Amano M, Ito M, Kimura K, Fukata Y, Chihara K, Nakano T . Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase). J Biol Chem. 1996; 271(34):20246-9. DOI: 10.1074/jbc.271.34.20246. View

Davis M, Wu X, Nurkiewicz T, Kawasaki J, Gui P, Hill M . Regulation of ion channels by protein tyrosine phosphorylation. Am J Physiol Heart Circ Physiol. 2001; 281(5):H1835-62. DOI: 10.1152/ajpheart.2001.281.5.H1835. View

Wier W, Morgan K . Alpha1-adrenergic signaling mechanisms in contraction of resistance arteries. Rev Physiol Biochem Pharmacol. 2003; 150:91-139. DOI: 10.1007/s10254-003-0019-8. View

Sakurada S, Takuwa N, Sugimoto N, Wang Y, Seto M, Sasaki Y . Ca2+-dependent activation of Rho and Rho kinase in membrane depolarization-induced and receptor stimulation-induced vascular smooth muscle contraction. Circ Res. 2003; 93(6):548-56. DOI: 10.1161/01.RES.0000090998.08629.60. View

Kahonen M, Karjala K, Hutri-Kahonen N, Wu X, Jaatinen P, Riihioja P . Influence of chronic ethanol consumption on arterial tone in young and aged rats. Am J Physiol. 1999; 276(2):H464-71. DOI: 10.1152/ajpheart.1999.276.2.H464. View

Muranyi A, MacDonald J, Deng J, Wilson D, Haystead T, Walsh M . Phosphorylation of the myosin phosphatase target subunit by integrin-linked kinase. Biochem J. 2002; 366(Pt 1):211-6. PMC: 1222775. DOI: 10.1042/BJ20020401. View

Fonseca B, Lee V, Proud C . The binding of PRAS40 to 14-3-3 proteins is not required for activation of mTORC1 signalling by phorbol esters/ERK. Biochem J. 2008; 411(1):141-9. DOI: 10.1042/BJ20071001. View

Gerthoffer W, Yamboliev I, Shearer M, Pohl J, Haynes R, Dang S . Activation of MAP kinases and phosphorylation of caldesmon in canine colonic smooth muscle. J Physiol. 1996; 495 ( Pt 3):597-609. PMC: 1160768. DOI: 10.1113/jphysiol.1996.sp021619. View

Tsai M, Jiang M . Rho-kinase-mediated regulation of receptor-agonist-stimulated smooth muscle contraction. Pflugers Arch. 2006; 453(2):223-32. DOI: 10.1007/s00424-006-0133-y. View

10.

Murphy R . Myosin phosphorylation and crossbridge regulation in arterial smooth muscle. State-or-the-art review. Hypertension. 1982; 4(3 Pt 2):3-7. View

11.

Nicoletti P, Trevisani M, Manconi M, Gatti R, De Siena G, Zagli G . Ethanol causes neurogenic vasodilation by TRPV1 activation and CGRP release in the trigeminovascular system of the guinea pig. Cephalalgia. 2007; 28(1):9-17. DOI: 10.1111/j.1468-2982.2007.01448.x. View

12.

Altura B, Altura B . Microvascular and vascular smooth muscle actions of ethanol, acetaldehyde, and acetate. Fed Proc. 1982; 41(8):2447-51. View

13.

Kitazawa T, Masuo M, Somlyo A . G protein-mediated inhibition of myosin light-chain phosphatase in vascular smooth muscle. Proc Natl Acad Sci U S A. 1991; 88(20):9307-10. PMC: 52703. DOI: 10.1073/pnas.88.20.9307. View

14.

Sahna E, Kurcer Z, Ozturk F, Cengiz N, Vardi N, Birincioglu M . Effects of chronic ethanol consumption on alpha-adrenergic-induced contractions and endothelium-dependent relaxations in rat thoracic aorta. Pharmacol Res. 2000; 41(6):629-33. DOI: 10.1006/phrs.1999.0629. View

15.

Han H, Du B, Pan X, Liu J, Zhao Q, Lian X . CADPE inhibits PMA-stimulated gastric carcinoma cell invasion and matrix metalloproteinase-9 expression by FAK/MEK/ERK-mediated AP-1 activation. Mol Cancer Res. 2010; 8(11):1477-88. DOI: 10.1158/1541-7786.MCR-10-0114. View

16.

LOW A . Role of tyrosine kinase on Ca2+ entry and refilling of agonist-sensitive Ca2+ stores in vascular smooth muscles. Can J Physiol Pharmacol. 1996; 74(3):298-304. View

17.

Somlyo A, Somlyo A . Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J Physiol. 2000; 522 Pt 2:177-85. PMC: 2269761. DOI: 10.1111/j.1469-7793.2000.t01-2-00177.x. View

18.

Nobe K, Paul R . Distinct pathways of Ca(2+) sensitization in porcine coronary artery: effects of Rho-related kinase and protein kinase C inhibition on force and intracellular Ca(2+). Circ Res. 2001; 88(12):1283-90. DOI: 10.1161/hh1201.092035. View

19.

Tasaki K, Hori M, Ozaki H, Karaki H, Wakabayashi I . Difference in signal transduction mechanisms involved in 5-hydroxytryptamine- and U46619-induced vasoconstrictions. J Smooth Muscle Res. 2003; 39(5):107-17. DOI: 10.1540/jsmr.39.107. View

20.

Somlyo A, Somlyo A . From pharmacomechanical coupling to G-proteins and myosin phosphatase. Acta Physiol Scand. 1999; 164(4):437-48. DOI: 10.1046/j.1365-201X.1998.00454.x. View