No-go Neurons in the Cerebellar Oculomotor Vermis and Caudal Fastigial Nuclei: Planning Tracking Eye Movements

The cerebellar dorsal vermis lobules VI-VII (oculomotor vermis) and its output region (caudal fastigial nuclei, cFN) are involved in tracking eye movements consisting of both smooth-pursuit and saccades, yet, the exact role of these regions in the control of tracking eye movements is still unclear. We compared the neuronal discharge of these cerebellar regions using a memory-based, smooth-pursuit task that distinguishes discharge related to movement preparation and execution from the discharge related to the processing of visual motion signals or their memory. Monkeys were required to pursue (i.e., go), or not pursue (i.e., no-go) in a cued direction, based on the memory of visual motion direction and go/no-go instructions. Most (>60 %) of task-related vermal Purkinje cells (P-cells) and cFN neurons discharged specifically during the memory period following no-go instructions; their discharge was correlated with memory of no-go instructions but was unrelated to eye movements per se during the action period of go trials. The latencies of no-go discharge of vermal P-cells and cFN neurons were similar, but were significantly longer than those of supplementary eye field (SEF) no-go neurons during an identical task. Movement-preparation signals were found in ~30 % of smooth-pursuit-related neurons in these cerebellar regions and some of them also carried visual memory signals. Our results suggest that no-go neurons are a newly revealed class of neurons, detected using the memory-based pursuit task, in the oculomotor vermis-cFN pathway and that this pathway contributes specifically to planning requiring the working memory of no-go instructions and preparation of tracking eye movements.